MGI phenotypes (allele matching)
B6.129S2-En1tm1(PDGFB)Nist/Kctt
| Status | Available to order |
| EMMA ID | EM:01289 |
| Citation information | RRID:IMSR_EM:01289 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129S2-En1tm1(PDGFB)Nist/Kctt |
| Alternative name | MNi-1 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | En1tm1(PDGFB)Nist |
| Gene/Transgene symbol | En1 |
Information from provider
| Provider | Monica NISTER |
| Provider affiliation | Department of Oncology and Pathology, Karolinska Institutet |
| Genetic information | Human platelet derived growth factor B (PDGFB), as well as an IRES-lacZ gene and floxed neo cassette replaced some 5' sequence of the coding region. Southern blot and PCR confirmed incorporation of the PDGFB coding sequence. In situ hybridization using human PDGFB probes also confirmed expression of the knock-in gene. |
| Phenotypic information | Affected cell migration in the midline during fusion of the cerebellar anlage, misplacement of midline structures and enhancement of capillary vessels. |
| Breeding history | The strain has been backcrossed to C57BL/6 since its establishment. |
| References |
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Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
Disease and phenotype information
MGI phenotypes (gene matching)
- delayed bone ossification / MGI
- increased cell proliferation / MGI
- polydactyly / MGI
- syndactyly / MGI
- truncation of digits / MGI
- ectopic digits / MGI
- abnormal digit pigmentation / MGI
- abnormal autopod morphology / MGI
- abnormal foot pad morphology / MGI
- abnormal nail morphology / MGI
- deformed nails / MGI
- abnormal eccrine sweat gland morphology / MGI
- increased inferior colliculus size / MGI
- abnormal choroid plexus morphology / MGI
- abnormal hindbrain morphology / MGI
- abnormal cerebellum morphology / MGI
- absent cerebellum / MGI
- abnormal cerebellum development / MGI
- abnormal cerebellum vermis morphology / MGI
- decreased anterior vermis size / MGI
- abnormal cerebellum posterior vermis morphology / MGI
- abnormal corpora quadrigemina morphology / MGI
- increased superior colliculus size / MGI
- abnormal brain development / MGI
- abnormal cranial nerve morphology / MGI
- absent oculomotor nerve / MGI
- absent trochlear nerve / MGI
- wrinkled skin / MGI
- decreased body size / MGI
- ataxia / MGI
- hyperactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- abnormal suckling behavior / MGI
- aphagia / MGI
- abnormal apical ectodermal ridge morphology / MGI
- thin apical ectodermal ridge / MGI
- increased dopamine level / MGI
- male infertility / MGI
- premature death / MGI
- abnormal limb morphology / MGI
- abnormal digit morphology / MGI
- abnormal axial skeleton morphology / MGI
- abnormal limb bone morphology / MGI
- abnormal brain morphology / MGI
- abnormal fertility/fecundity / MGI
- no abnormal phenotype detected / MGI
- abnormal innervation / MGI
- abnormal brain commissure morphology / MGI
- abnormal CNS synaptic transmission / MGI
- abnormal neuron morphology / MGI
- decreased superior colliculus size / MGI
- abnormal axon guidance / MGI
- abnormal cell cycle / MGI
- abnormal cell migration / MGI
- abnormal locomotor coordination / MGI
- decreased rib number / MGI
- nervous system phenotype / MGI
- abnormal hair follicle development / MGI
- abnormal hair follicle dermal papilla morphology / MGI
- abnormal midbrain development / MGI
- abnormal serotonergic neuron morphology / MGI
- short sternum / MGI
- abnormal sternebra morphology / MGI
- abnormal brain vasculature morphology / MGI
- behavior/neurological phenotype / MGI
- skeleton phenotype / MGI
- abnormal cerebellar lobule formation / MGI
- absent cerebellar lobules / MGI
- asymmetric sternocostal joints / MGI
- abnormal sternum ossification / MGI
- fused phalanges / MGI
- decreased physiological sensitivity to xenobiotic / MGI
- decreased inferior colliculus size / MGI
- absent inferior colliculus / MGI
- abnormal synapse morphology / MGI
- abnormal neuron differentiation / MGI
- decreased serotonin level / MGI
- mortality/aging / MGI
- postnatal lethality, incomplete penetrance / MGI
- neonatal lethality, complete penetrance / MGI
- perinatal lethality, complete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- decreased dopaminergic neuron number / MGI
- ectopic dopaminergic neuron / MGI
- decreased midbrain size / MGI
- decreased rhombomere 1 size / MGI
Literature references
- Forced expression of platelet-derived growth factor B in the mouse cerebellar primordium changes cell migration during midline fusion and causes cerebellar ectopia.;Andrae Johanna, Afink Gijs, Zhang Xiao-Qun, Wurst Wolfgang, Nistér Monica, ;2004;Molecular and cellular neurosciences;26;308-21; 15207855