B6(Cg)-Cacna1btm1.1Arte/H

Status

Available to order

EMMA IDEM:13038
Citation informationRRID:IMSR_EM:13038 

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International strain nameB6(Cg)-Cacna1btm1.1Arte/H
Alternative nameCaV2.2_HA Knockin
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolCacna1bKI-HA
Gene/Transgene symbolCacna1b

Information from provider

ProviderAnnette Dolphin
Provider affiliationNPP, UCL
Genetic informationConstitutive knock-in of a 2xHA tag in the Cacna1b (CaV2.2) gene.
Phenotypic informationHomozygous:
Homozygous CaV2.2_HA knock-in mice are not different phenotypically from wild-type mice but provide a tool to visualise endogenous CaV2.2 channels.

Heterozygous:
Heterozygous CaV2.2_HA knock-in mice are not different phenotypically from wild-type mice
Breeding historyMutant mice generated by TaconicArtemis in 2014 and backcrossed to wild-type C57BL/6J several times since.
References
  • Ablation of α2δ-1 inhibits cell-surface trafficking of endogenous N-type calcium channels in the pain pathway in vivo.;Nieto-Rostro Manuela, Ramgoolam Krishma, Pratt Wendy S, Kulik Akos, Dolphin Annette C, ;2018;Proceedings of the National Academy of Sciences of the United States of America;115;E12043-E12052; 30487217
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • hyperactivity / IMPC
  • abnormal gait / IMPC
  • abnormal locomotor behavior / IMPC
  • abnormal behavior / IMPC
  • decreased grip strength / IMPC
  • decreased thigmotaxis / IMPC
  • decreased fasting circulating glucose level / IMPC
  • decreased anxiety-related response / IMPC
  • increased exploration in new environment / IMPC
MGI phenotypes (gene matching)
  • hypertension / MGI
  • increased aggression towards mice / MGI
  • decreased anxiety-related response / MGI
  • hyperactivity / MGI
  • abnormal spatial learning / MGI
  • reduced long term potentiation / MGI
  • decreased startle reflex / MGI
  • abnormal sleep pattern / MGI
  • abnormal lipid level / MGI
  • abnormal hypothalamus secretion / MGI
  • increased thermal nociceptive threshold / MGI
  • decreased chemically-elicited antinociception / MGI
  • abnormal learning/memory/conditioning / MGI
  • abnormal sensory capabilities/reflexes/nociception / MGI
  • abnormal nervous system electrophysiology / MGI
  • alcohol aversion / MGI
  • increased heart rate / MGI
  • decreased circulating triglyceride level / MGI
  • decreased circulating glucagon level / MGI
  • decreased circulating insulin level / MGI
  • abnormal discrimination learning / MGI
  • abnormal conditioned taste aversion behavior / MGI
  • abnormal excitatory postsynaptic potential / MGI
  • abnormal inhibitory postsynaptic currents / MGI
  • impaired social transmission of food preference / MGI
  • abnormal frequency of paradoxical sleep / MGI
  • abnormal single cell response / MGI
  • abnormal summary potential / MGI
  • abnormal channel response / MGI
  • decreased alcohol consumption / MGI
  • nervous system phenotype / MGI
  • abnormal synaptic transmission / MGI
  • abnormal sympathetic nervous system physiology / MGI
  • abnormal baroreceptor physiology / MGI
  • abnormal miniature excitatory postsynaptic currents / MGI
  • improved glucose tolerance / MGI
  • homeostasis/metabolism phenotype / MGI
  • growth/size/body region phenotype / MGI
  • endocrine/exocrine gland phenotype / MGI
  • behavior/neurological phenotype / MGI
  • hyporesponsive to tactile stimuli / MGI
  • decreased susceptibility to diet-induced obesity / MGI
  • decreased circulating leptin level / MGI
  • abnormal long term spatial reference memory / MGI
  • increased chemical nociceptive threshold / MGI
  • abnormal miniature inhibitory postsynaptic currents / MGI
  • fragmentation of sleep/wake states / MGI
  • impaired conditioned place preference behavior / MGI
  • abnormal behavioral response to xenobiotic / MGI
  • enhanced behavioral response to alcohol / MGI
  • impaired behavioral response to alcohol / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI

Literature references

  • Ablation of α2δ-1 inhibits cell-surface trafficking of endogenous N-type calcium channels in the pain pathway in vivo.;Nieto-Rostro Manuela, Ramgoolam Krishma, Pratt Wendy S, Kulik Akos, Dolphin Annette C, ;2018;Proceedings of the National Academy of Sciences of the United States of America;115;E12043-E12052; 30487217

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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