B6J;129-Sod1tm1.1(SOD1)Emcf/H
| Status | Available to order |
| EMMA ID | EM:13075 |
| Citation information | RRID:IMSR_EM:13075 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J;129-Sod1tm1.1(SOD1)Emcf/H |
| Alternative name | C57BL/6J-Sod1 tm1.1(SOD1)Emcf/H |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Sod1tm1.1(SOD1)Emcf |
| Gene/Transgene symbol | Sod1 |
Information from provider
| Provider | Elizabeth Fisher |
| Provider affiliation | Department of Neuromuscular Diseases, UCL |
| Genetic information | C57BL/6J-Sod1tm1.1(SOD1)Emcf/H knock-in mice carry the human version of the Cu,Zn-superoxide dismutase-1 (SOD1) gene, which replaces the mouse Sod1 gene at the endogenous locus. The humanized region begins at the first codon in exon 1 of the Sod1 gene, and continues through to the end of the human SOD1 5’ UTR. Exons 4, 5 and the 5’ UTR of the humanized Sod1 gene have been floxed and duplicated to allow conditional expression of a second copy of exons 4 and 5. These mice were generated using homologous recombination in R1 ES cells. |
| Phenotypic information | Homozygous:Homozygous mice are viable, fertile and produced at normal mendelian ratios. Homozygous mice have no overt phenotype (comprehensive phenotyping has not yet been conducted). The oldest homozygous C57BL/6J-Sod1 |
| Breeding history | Targeting was performed in R1 ES cells, followed by injection into CD1 blastocysts. Chimeric offspring were crossed to C57BL/6J and founder C57BL/6J-Sod1tm1(SOD1)Emcf/H mice carrying the humanized allele were selected and crossed to C57BL/6J for one subsequent generation. C57BL/6J-Sod1tm1(SOD1)Emcf/H embryos were harvested from superovulated females, underwent IVF with C57BL/6J sperm and cytoplasmic injection of Flpo mRNA to remove the neomycin selection cassette. C57BL/6J-Sod1tm1.1(SOD1)Emcf/H mice carrying human SOD1 with the neomycin selection cassette excised were selected and backcrossed onto C57BL/6J for 2 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Amyotrophic lateral sclerosis / Orphanet_803
Literature references
- Generation and analysis of innovative genomically humanized knockin SOD1, TARDBP (TDP-43), and FUS mouse models.;Devoy Anny, Price Georgia, De Giorgio Francesca, Bunton-Stasyshyn Rosie, Thompson David, Gasco Samanta, Allan Alasdair, Codner Gemma F, Nair Remya R, Tibbit Charlotte, McLeod Ross, Ali Zeinab, Noda Judith, Marrero-Gagliardi Alessandro, Brito-Armas José M, Williams Chloe, Öztürk Muhammet M, Simon Michelle, O'Neill Edward, Bryce-Smith Sam, Harrison Jackie, Atkins Gemma, Corrochano Silvia, Stewart Michelle, Gilthorpe Jonathan D, Teboul Lydia, Acevedo-Arozena Abraham, Fisher Elizabeth M C, Cunningham Thomas J, ;2021;iScience;24;103463; 34988393