B6(Cg)-Zfp503^tm1.1Pnmn/H

Status	Available to order
EMMA ID	EM:13089
Citation information	RRID:IMSR_EM:13089 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6(Cg)-Zfp503^tm1.1Pnmn/H
Alternative name	Nolz1fl/fl
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Zfp503^tm1.1Pnmn
Gene/Transgene symbol	Zfp503

Information from provider

Provider	Lia Panman
Provider affiliation	MRC Toxicology Unit, University of Cambridge
Genetic information	We generated a conditional knock-out allele by inserting loxP sites flanking exon3 (coding region) of Zfp503 (Nolz1) by homologous recombination in ES cells.
Phenotypic information	Homozygous: Nolz1fl/fl homozygous mutant animals do not display any phenotype. Upon cre recombinase-mediated recombination Nolz1 expression is ablated in specific areas of the embryo. Total ablation of Nolz1 expression by using a CMV-cre line results in death immediately after birth. Forebrain selective inactivation of Nolz1 results in striatal patterning defects and misguidance of several axonal tracts including dopaminergic and thalamocortical projections. Heterozygous: Embryos/mice heterozygous or hemizygous for conditional Nolz1fl allele do not display any phenotype.
Breeding history	The mouse line has been crossed with other animals on a C57BL/6J background. Colony was initially established by crossing heterozygous animals with C57BL/6J wild-type animals for 4 generations. Thereafter, the colony has been maintained as a homozygous colony for at least 8 generations.
References	Nolz1 expression is required in dopaminergic axon guidance and striatal innervation.;Soleilhavoup Clement, Travaglio Marco, Patrick Kieran, Garção Pedro, Boobalan Elangovan, Adolfs Youri, Spriggs Ruth V, Moles-Garcia Emma, Dhiraj Dalbir, Oosterveen Tony, Ferri Sarah L, Abel Ted, Brodkin Edward S, Pasterkamp R Jeroen, Brooks Brian P, Panman Lia, ;2020;Nature communications;11;3111; 32561725
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal lymph node morphology / IMPC
edema / IMPC
decreased circulating creatinine level / IMPC
enlarged lymph nodes / IMPC
decreased grip strength / IMPC
embryonic growth retardation / IMPC
decreased circulating glucose level / IMPC
preweaning lethality, complete penetrance / IMPC
anophthalmia / IMPC
abnormal kidney morphology / IMPC
microcephaly / IMPC
increased basophil cell number / IMPC
increased exploration in new environment / IMPC
abnormal embryo size / IMPC
abnormal male genitalia morphology / IMPC
abnormal optic disk morphology / IMPC
enlarged kidney / IMPC

Literature references

Nolz1 expression is required in dopaminergic axon guidance and striatal innervation.;Soleilhavoup Clement, Travaglio Marco, Patrick Kieran, Garção Pedro, Boobalan Elangovan, Adolfs Youri, Spriggs Ruth V, Moles-Garcia Emma, Dhiraj Dalbir, Oosterveen Tony, Ferri Sarah L, Abel Ted, Brodkin Edward S, Pasterkamp R Jeroen, Brooks Brian P, Panman Lia, ;2020;Nature communications;11;3111; 32561725

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B6(Cg)-Zfp503tm1.1Pnmn/H