IMPC phenotypes (gene matching)
B6.Cg-Marcotm1Ktry Msr1tm1Csk/H
| Status | Available to order |
| EMMA ID | EM:13143 |
| Citation information | RRID:IMSR_EM:13143 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Marcotm1Ktry Msr1tm1Csk/H |
| Alternative name | Msr1-Marco double knockout mice |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Msr1tm1Csk, Marcotm1Ktry |
| Gene/Transgene symbol | Msr1, Marco |
Information from provider
| Provider | Karl Tryggvason |
| Provider affiliation | Duke-NUS Medical School |
| Additional owner | Prof. Siamon Gordon, Department of Pathology, University of Oxford, United Kingdom |
| Genetic information | Msr1 knock-out mice and Marco knock-out mice in C57BL/6J background are crossed together to generate Msr1-Marco double knock-out mice. |
| Phenotypic information | Homozygous:Lack the MARCO protein in selected macrophage populations. Defective spleen marginal zone architecture. Unable to induce T independent antigen response. Susceptible to variety of bacterial fungal and viral infection.Heterozygous:Same as wild-type. |
| Breeding history | Msr1 and Marco single knock-out mice were backcrossed to C57BL/6J line for 10 generations and each established homozygous line has been maintained as brother x sister mate. The single knock-out mice are crossed to generate homozygous Msr1 and Marco double knock-out mice. The double knock-out strain breeds just as good as the background strain with average litter sizes of 5/7 pups andthe health status has remained consistent for many years. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (allele matching)
MGI phenotypes (gene matching)
- impaired macrophage phagocytosis / MGI
- increased inflammatory response / MGI
- abnormal spleen marginal zone morphology / MGI
- abnormal dendritic cell physiology / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal macrophage physiology / MGI
- increased interferon-gamma secretion / MGI
- increased sensitivity to induced morbidity/mortality / MGI
- abnormal dendritic cell chemotaxis / MGI
Literature references
- Defective microarchitecture of the spleen marginal zone and impaired response to a thymus-independent type 2 antigen in mice lacking scavenger receptors MARCO and SR-A.;Chen Yunying, Pikkarainen Timo, Elomaa Outi, Soininen Raija, Kodama Tatsuhiko, Kraal Georg, Tryggvason Karl, ;2005;Journal of immunology (Baltimore, Md. : 1950);175;8173-80; 16339556
- A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection.;Suzuki H, Kurihara Y, Takeya M, Kamada N, Kataoka M, Jishage K, Ueda O, Sakaguchi H, Higashi T, Suzuki T, Takashima Y, Kawabe Y, Cynshi O, Wada Y, Honda M, Kurihara H, Aburatani H, Doi T, Matsumoto A, Azuma S, Noda T, Toyoda Y, Itakura H, Yazaki Y, Kodama T, ;1997;Nature;386;292-6; 9069289
- The macrophage scavenger receptor type A is expressed by activated macrophages and protects the host against lethal endotoxic shock.;Haworth R, Platt N, Keshav S, Hughes D, Darley E, Suzuki H, Kurihara Y, Kodama T, Gordon S, ;1997;The Journal of experimental medicine;186;1431-9; 9348300
- SR-A/MARCO-mediated ligand delivery enhances intracellular TLR and NLR function, but ligand scavenging from cell surface limits TLR4 response to pathogens.;Mukhopadhyay Subhankar, Varin Audrey, Chen Yunying, Liu Baoying, Tryggvason Karl, Gordon Siamon, ;2011;Blood;117;1319-28; 21098741