B6.Cg-Lgals1^tm1Rob/JCnbc

Status	Available to order
EMMA ID	EM:13160
Citation information	RRID:IMSR_EM:13160 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Lgals1^tm1Rob/JCnbc
Alternative name	B6.Cg-Lgals1tm1Rob/J
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Lgals1^tm1Rob
Gene/Transgene symbol	Lgals1

Information from provider

Provider	Pilar Navarro
Provider affiliation	Cell death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB)-CSIC
Additional owner	Dr. Elizabeth Robertson, Medical Sciences Division, University of Oxford, UK
Genetic information	Lgals1 (Gal-1; Lect14) knock-out mice were obtained by kind gift of Dr. F. Poirier (Poirier F. and Robertson E. Development 119, 1229-1236; 1993). They were generated by targeted mutation. In particular, exon 2, encoding the carbohydrate-binding domain, of Lgals1 locus was replaced with a neomycin selection cassette. Western blot analysis and immunostaining of sections showed an absence of encoded protein in muscle tissue obtained from adult homozygous mutant mice.
Phenotypic information	Homozygous: We did not detect any abnormalities in the breeding or viability of these mice. Alteration of the mouse olfactory system has been reported in other genetic backgrounds (129S/SvEv * MF1; Puche AC. et al., Dev Biol 1996). In particular, while olfactory nerve itself was normal, projections from it to the dorsocaudal surface of the olfactory bulb were severely reduced and axons never terminated in glomeruli, but we have not analysed the phenotype at this level in our C57BL/6J genetic background. Heterozygous: normal
Breeding history	Original background of the Gal-1 -/- mice was 129Sv (original background strain from Françoise Poirier). Dr. Poirier later generated them into a C57BL/6J background by backcrossing to C57BL/6J wild-type mice. We have maintained these mice into this genetic background by inbred crossing (B6 Gal-1 -/- x B6 Gal-1 -/-) as well as periodic breeding with C57BL/6J wild-type (once a year, to avoid excessive inbreeding).
References	Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin.;Poirier F, Robertson E J, ;1993;Development (Cambridge, England);119;1229-36; 8306885 Galectin-1 drives pancreatic carcinogenesis through stroma remodeling and Hedgehog signaling activation.;Martínez-Bosch Neus, Fernández-Barrena Maite G, Moreno Mireia, Ortiz-Zapater Elena, Munné-Collado Jessica, Iglesias Mar, André Sabine, Gabius Hans-Joachim, Hwang Rosa F, Poirier Françoise, Navas Carolina, Guerra Carmen, Fernández-Zapico Martin E, Navarro Pilar, ;2014;Cancer research;74;3512-24; 24812270 Targeting galectin-1 inhibits pancreatic cancer progression by modulating tumor-stroma crosstalk.;Orozco Carlos A, Martinez-Bosch Neus, Guerrero Pedro E, Vinaixa Judith, Dalotto-Moreno Tomás, Iglesias Mar, Moreno Mireia, Djurec Magdolna, Poirier Françoise, Gabius Hans-Joachim, Fernandez-Zapico Martin E, Hwang Rosa F, Guerra Carmen, Rabinovich Gabriel A, Navarro Pilar, ;2018;Proceedings of the National Academy of Sciences of the United States of America;115;E3769-E3778; 29615514
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous C57BL/6J males, homozygous C57BL/6J females
Stage of embryos	2-cell

Disease and phenotype information

MGI phenotypes (gene matching)

delayed muscle development / MGI
abnormal dorsal root ganglion morphology / MGI
abnormal sensory neuron morphology / MGI
abnormal nociceptor morphology / MGI
abnormal mechanoreceptor morphology / MGI
abnormal neuromuscular synapse morphology / MGI
increased anxiety-related response / MGI
increased thermal nociceptive threshold / MGI
abnormal social/conspecific interaction / MGI
abnormal cytokine secretion / MGI
abnormal postnatal subventricular zone morphology / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
abnormal olfactory nerve morphology / MGI
homeostasis/metabolism phenotype / MGI
behavior/neurological phenotype / MGI
reproductive system phenotype / MGI
abnormal olfactory sensory neuron morphology / MGI
increased interferon-gamma secretion / MGI
increased interleukin-2 secretion / MGI
mortality/aging / MGI

Literature references

Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin.;Poirier F, Robertson E J, ;1993;Development (Cambridge, England);119;1229-36; 8306885
Galectin-1 drives pancreatic carcinogenesis through stroma remodeling and Hedgehog signaling activation.;Martínez-Bosch Neus, Fernández-Barrena Maite G, Moreno Mireia, Ortiz-Zapater Elena, Munné-Collado Jessica, Iglesias Mar, André Sabine, Gabius Hans-Joachim, Hwang Rosa F, Poirier Françoise, Navas Carolina, Guerra Carmen, Fernández-Zapico Martin E, Navarro Pilar, ;2014;Cancer research;74;3512-24; 24812270
Targeting galectin-1 inhibits pancreatic cancer progression by modulating tumor-stroma crosstalk.;Orozco Carlos A, Martinez-Bosch Neus, Guerrero Pedro E, Vinaixa Judith, Dalotto-Moreno Tomás, Iglesias Mar, Moreno Mireia, Djurec Magdolna, Poirier Françoise, Gabius Hans-Joachim, Fernandez-Zapico Martin E, Hwang Rosa F, Guerra Carmen, Rabinovich Gabriel A, Navarro Pilar, ;2018;Proceedings of the National Academy of Sciences of the United States of America;115;E3769-E3778; 29615514

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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B6.Cg-Lgals1tm1Rob/JCnbc