IMPC phenotypes (gene matching)
STOCK Hint2tm1.1Geno Hint1tm1Ibw/Orl
| Status | Available to order |
| EMMA ID | EM:13167 |
| Citation information | RRID:IMSR_EM:13167 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Hint2tm1.1Geno Hint1tm1Ibw/Orl |
| Alternative name | Hint1xHint2 KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Hint1tm1Ibw, Hint2tm1.1Geno |
| Gene/Transgene symbol | Hint1, Hint2 |
Information from provider
| Provider | Jean-Francois Dufour |
| Provider affiliation | DBMR Hepatology, University of Bern |
| Additional owner | Hint1 strain was a gift from Prof. Weinstein, NY USA |
| Genetic information | Hint1 knock-out: ES cells heterozygous for the targeted mutation were microinjected into C57BL/6 blastocysts and implanted into pseudopregnant females. The resulting male chimeras were mated to C57BL/6 female mice. Germ-line transmission of the injected ES cells was confirmed by inheritance of the agouti coat color in F1 mice, and the agouti offspring were tested for the presence of the mutated Hint1 allele by Southern blot analysis of mouse tail DNA. These mice were backcrossed to mice of 129 strain background for several generations, so that in the Hint1-/- mice used in the present study over 96% of their background is from the 129 strain. Hint2 knock-out: cre recombinase-mediated excision of the floxed Hint2 gene, deletion of exon 1 to exon 5. |
| Phenotypic information | Homozygous:When homozygous, Hint1 knock-out mice show higher incidence in tumours. Hint2 knock-out homozygotes show no evident phenotype. Heterozygous:Hint1 is a haplo-insufficient tumour suppressor. Hint2 heterozygotes show no evident phenotype. |
| Breeding history | This strain was obtained by crossing the Hint1 knock-out strain with the Hint2 knock-out strain. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous 129 x C57BL/6 males, homozygous 129 x C57BL/6 females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autosomal recessive axonal neuropathy with neuromyotonia / Orphanet_324442
Literature references
- Hint1 is a haplo-insufficient tumor suppressor in mice.;Li H, Zhang Y, Su T, Santella R M, Weinstein I B, ;2006;Oncogene;25;713-21; 16186798
- Deletion of histidine triad nucleotide-binding protein 1/PKC-interacting protein in mice enhances cell growth and carcinogenesis.;Su Tao, Suzui Masumi, Wang Lei, Lin Chyuan-Sheng, Xing Wang-Qiu, Weinstein I Bernard, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;7824-9; 12810953
- Ablation of the tumor suppressor histidine triad nucleotide binding protein 1 is protective against hepatic ischemia/reperfusion injury.;Martin Juliette, Romanque Pamela, Maurhofer Olivier, Schmitter Karin, Hora Caroline, Ferrand Gisèle, Dufour Jean-François, ;2011;Hepatology (Baltimore, Md.);53;243-52; 21140474
- Disruption of tumor suppressor gene Hint1 leads to remodeling of the lipid metabolic phenotype of mouse liver.;Beyoğlu Diren, Krausz Kristopher W, Martin Juliette, Maurhofer Olivier, Dorow Juliane, Ceglarek Uta, Gonzalez Frank J, Dufour Jean-François, Idle Jeffrey R, ;2014;Journal of lipid research;55;2309-19; 25193995