B6.Cg-Tatdn1tm1.1Csaag/Cnbc
| Status | Available to order |
| EMMA ID | EM:13201 |
| Citation information | RRID:IMSR_EM:13201 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tatdn1tm1.1Csaag/Cnbc |
| Alternative name | tatdn1 floxed |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Tatdn1tm1.1Csaag |
| Gene/Transgene symbol | Tatdn1 |
Information from provider
| Provider | Daniel Sanchis |
| Provider affiliation | Basic Medical Sciences, University of LLeida |
| Genetic information | Tatdn1 loxP/loxP was designed and produced by ARMI/MONASH, Australia. Tatdn1 exon 3 was flanked by loxP sites. Deletion of exon 3 induces a frameshift and stop codon in exon 4, giving a protein of 40 amino acids. The neomycin resistance cassette for ES selection was originally flanked by FRT sites (FRT-PGK-em7-Neomycin-pA-FRT). Male chimeras were crossed with C57BL/6 females in 2011. Later, we crossed Tatdn1 transgenic mice with a Gt(ROSA)26Sor-FLPe strain, inducing deletion of the NeoR cassette and the present Tatdn1 loxP/loxP mice lack the NeoR gene. |
| Phenotypic information | Homozygous:Fertility and embryonic development seem not affected. Deletion of Tatdn1 by further crossing with cre recombinase-expressing mice produces normal fertile mice, breeding at the expected number of male and female descendants. Normal gross morphology. We have analysed heart function and behaviour. Mild ventricular hypertrophy and potential locomotor alterations are induced by Tatdn1 deletion. Heterozygous:Not assessed. |
| Breeding history | Tatdn1 loxP FRT male chimeras were crossed with C57BL/6 wild type females to obtain the F1 mice in 2011. Floxed mice were crossed for several generations within the C57BL/6 background. FRT deletion was generated by crossing with the Gt(ROSA)26Sor-FLPe strain from the Jackson Lab. (B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ) in 2013. Since then, animals have been backcrossed in a C57BL/6 background many times. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Mammalian TatD DNase domain containing 1 (TATDN1) is a proteostasis-responsive gene with roles in ventricular structure and neuromuscular function.;Barés Gisel, Beà Aida, Sancho-Balsells Anna, Valero Juan G, Aluja David, Inserte Javier, García-Carpi Sandra, Miró-Casas Elisabet, Borràs-Pernas Sara, Hernández Sara, Martínez-Val Ana, Olsen Jesper V, Tebar Francesc, Cañas Xavier, Comella Joan X, Pérez-Galán Patricia, Ruiz-Meana Marisol, Giralt Albert, Llovera Marta, Sanchis Daniel, ;2025;The FEBS journal;292;5105-5129; 40123200