IMPC phenotypes (gene matching)
B6.Cg-Pik3c3tm1Bvan/H
| Status | Available to order |
| EMMA ID | EM:13251 |
| Citation information | RRID:IMSR_EM:13251 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Pik3c3tm1Bvan/H |
| Alternative name | Vps34-flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Pik3c3tm1Bvan |
| Gene/Transgene symbol | Pik3c3 |
Information from provider
| Provider | Bart Vanhaesebroeck |
| Provider affiliation | University College London |
| Genetic information | Exon 21 of the kinase domain of the Pik3c3 (Vps34) gene was flanked by loxP sites. This exon encodes a critical stretch of 25 amino acids (Ala730 to Thr754) in the VPS34 kinase domain, producing an in-frame deletion upon Cre-mediated recombination, generating a truncated version of VPS34 which is catalytically inactive (Supplementary Fig S1A, B in Frontiers in Immunology;15;1374621; PMID: 39664390). |
| Phenotypic information | Homozygous:Mice are phenotypically normal.Heterozygous:Mice are phenotypically normal. |
| Breeding history | Backcrossed onto C57BL/6J for more than 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased cell proliferation / MGI
- absent mesoderm / MGI
- failure of primitive streak formation / MGI
- failure to gastrulate / MGI
- decreased embryo size / MGI
- embryonic growth arrest / MGI
- no abnormal phenotype detected / MGI
- abnormal embryonic epiblast morphology / MGI
- embryonic growth retardation / MGI
- disorganized embryonic tissue / MGI
- cellular phenotype / MGI
- embryonic lethality between implantation and placentation, complete penetrance / MGI
- abnormal visceral endoderm morphology / MGI
Literature references
- Vps34/PI3KC3 deletion in kidney proximal tubules impairs apical trafficking and blocks autophagic flux, causing a Fanconi-like syndrome and renal insufficiency.;Grieco Giuseppina, Janssens Virginie, Gaide Chevronnay Héloïse P, N'Kuli Francisca, Van Der Smissen Patrick, Wang Tongsong, Shan Jingdong, Vainio Seppo, Bilanges Benoit, Jouret François, Vanhaesebroeck Bart, Pierreux Christophe E, Courtoy Pierre J, ;2018;Scientific reports;8;14133; 30237523
- A dual role for the class III PI3K, Vps34, in platelet production and thrombus growth.;Valet Colin, Levade Marie, Chicanne Gaëtan, Bilanges Benoit, Cabou Cendrine, Viaud Julien, Gratacap Marie-Pierre, Gaits-Iacovoni Frédérique, Vanhaesebroeck Bart, Payrastre Bernard, Severin Sonia, ;2017;Blood;130;2032-2042; 28903944
- Lack of phosphatidylinositol 3-kinase VPS34 in regulatory T cells leads to a fatal lymphoproliferative disorder without affecting their development.;Courreges Christina J F, Davenport Elizabeth C M, Bilanges Benoit, Rebollo-Gomez Elena, Hukelmann Jens, Schoenfelder Priya, Edgar James R, Sansom David, Scudamore Cheryl L, Roychoudhuri Rahul, Garden Oliver A, Vanhaesebroeck Bart, Okkenhaug Klaus, ;2024;Frontiers in immunology;15;1374621; 39664390