B6;129P2-Igdmrtm1Ace/H
| Status | Available to order |
| EMMA ID | EM:13315 |
| Citation information | RRID:IMSR_EM:13315 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Igdmrtm1Ace/H |
| Alternative name | Igdmrtm1Ace |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Igdmrtm1Ace |
| Gene/Transgene symbol | Igdmr |
Information from provider
| Provider | Anne Ferguson-Smith |
| Provider affiliation | Genetics, University of Cambridge |
| Genetic information | Targeted mutation of Igdmr (intergenic differentially methylated region) on chromosome 12. The region was replaced by a floxed neomycin resistance cassette, which was subsequently excised via transient cre recombinase expression. Southern blot indicated successful targeting. The maternally inherited deletion results in biallelic expression of Dlk1 and Rtl1, with maternal transcript levels of Rtl1 at ~77% that of the unmanipulated paternal allele. The maternally inherited deletion also resulted in the activation of maternal Dio3. |
| Phenotypic information | Homozygous:Not viableHeterozygous:The maternally inherited deletion results in biallelic expression of Dlk1 and Rtl1, with maternal transcript levels of Rtl1 at ~77% that of the unmanipulated paternal allele. The maternally inherited deletion also resulted in the activation of maternal Dio3. |
| Breeding history | The line is maintained by breeding heterozygous males with C57BL/6 females. Maternal transmission of the mutation is lethal around E16.5. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12.;Lin Shau-Ping, Youngson Neil, Takada Shuji, Seitz Hervé, Reik Wolf, Paulsen Martina, Cavaille Jerome, Ferguson-Smith Anne C, ;2003;Nature genetics;35;97-102; 12937418