B6J(Cg)-Tg(CAG-SQSTM1)/H
| Status | Available to order |
| EMMA ID | EM:13523 |
| Citation information | RRID:IMSR_EM:13523 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J(Cg)-Tg(CAG-SQSTM1)/H |
| Alternative name | P62-2 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-SQSTM1) |
| Gene/Transgene symbol | Tg(CAG-SQSTM1) |
Information from provider
| Provider | David Rubinsztein |
| Provider affiliation | Medical Genetics, University of Cambridge |
| Genetic information | sequestosome 1 (SQSTM1 or p62) transgenic; the plasmid used, pCAGGS-p62, was created by taking p62 (without the tomato tag) with PCR from the pDest-tdTomato-p62 plasmid coming from Terje Johansen's lab (see "p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death", https://rupress.org/jcb/article/171/4/603/52022/) and cloning it into the pCAGGS vector (https://ecoliwiki.org/colipedia/index.php/pCAGGS). The cloning was done using the EcoRI site. DNA was injected and born mice were checked for p62 expression. |
| Phenotypic information | Homozygous:to be performedHeterozygous:to be performed |
| Breeding history | heterozygous x wild-type |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |