B6J.B6N-H2ajem1Cmnn/Orl
| Status | Available to order |
| EMMA ID | EM:13661 |
| Citation information | RRID:IMSR_EM:13661 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.B6N-H2ajem1Cmnn/Orl |
| Alternative name | H2afjDel7 (B6JRj) |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | H2ajem1Cmnn |
| Gene/Transgene symbol | H2aj |
Information from provider
| Provider | Carl MANN |
| Provider affiliation | Genome Biology, CEA/Saclay |
| Genetic information | TALEN-mediated 7-nucleotide deletion at the beginning of the H2aj (or H2afj) gene. The same targeted mutation (H2afj∆7) was back-crossed 6 times to either C57BL/6N (EM:13660) or C57BL/6J (EM:13661) as there are a few crucial differences between these 2 substrains. In particular, B6J carries an Nnt∆ mutation that makes the mice particularly susceptible to a high-fat diet and it is often used for such studies. The B6N substrain does not carry this mutation (but it has other mutations not found in the B6J substrain). |
| Phenotypic information | Homozygous:Homozygous mutant is viable and fertile. Phenotypic characterization is ongoing. The mutant gains weight less quickly than the isogenic wild-type control and may have lower levels of expression of some inflammatory genes. The mutant may show some protection from deleterious phenotypes induced by hat fat diet (in the C57BL/6J background) and ageing.Heterozygous:Heterozygous mutant is viable and fertile. No further phenotypic characterization was done with the heterozygous mutant. |
| Breeding history | The original heterozygous mutation was a TALEN-mediated 7-nucleotide deletion near the beginning of the H2afj gene in C57BL/6N (Charles River) created by Cyagen. It was backcrossed 6 times with C57BL/6JRj from Janvier Labs. The homozygous mutant is viable and fertile and the strain has been maintained as a homozygote. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous males |
Literature references
- Human skin aging is associated with increased expression of the histone variant H2A.J in the epidermis.;Rübe Claudia E, Bäumert Caroline, Schuler Nadine, Isermann Anna, Schmal Zoé, Glanemann Matthias, Mann Carl, Scherthan Harry, ;2021;NPJ aging and mechanisms of disease;7;7; 33795696
- Histone variant H2A.J accumulates in senescent cells and promotes inflammatory gene expression.;Contrepois Kévin, Coudereau Clément, Benayoun Bérénice A, Schuler Nadine, Roux Pierre-François, Bischof Oliver, Courbeyrette Régis, Carvalho Cyril, Thuret Jean-Yves, Ma Zhihai, Derbois Céline, Nevers Marie-Claire, Volland Hervé, Redon Christophe E, Bonner William M, Deleuze Jean-François, Wiel Clotilde, Bernard David, Snyder Michael P, Rübe Claudia E, Olaso Robert, Fenaille François, Mann Carl, ;2017;Nature communications;8;14995; 28489069