| Status | Available to order |
| EMMA ID | EM:01373 |
| Citation information | RRID:IMSR_EM:01373 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Sema3etm1Ddg/Orl |
| Alternative name | Sema3E |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sema3etm1Ddg |
| Gene/Transgene symbol | Sema3e |
| Provider | Christopher Henderson |
| Provider affiliation | INSERM UMR 623 - IBDM |
| Genetic information | The first coding exon of mouse Sema3e encodes the signal peptide. In the targeting vector, a DNA fragment encoding the green fluorescent protein EGFP is inserted exactly at the start codon of the Sema3e gene. The vector also contains a neo cassette flanked by loxP sites for ES cell selection. This vector was used to target the Sema3e locus by homologous recombination in ES cells and generate the mutant locus. Recombinant ES cells were injected into C57BL/6 blastocysts. Chimeric founders were bred to C57BL/6 mice, and heterozygous mice were crossed with cre recombinase-expressing mice in order to delete the neo-cassette. Finally heterozygous mice were used to establish lines that were backcrossed to either a C57BL/6 or a CD1 genetic background. |
| Phenotypic information | nothing |
| Breeding history | Five backcrosses to C57BL/6. |
| References |
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| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
