B6J;129S-Grb10tm1.1Fuis/H

Status

Available to order

EMMA IDEM:13738
Citation informationRRID:IMSR_EM:13738 

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International strain nameB6J;129S-Grb10tm1.1Fuis/H
Alternative nameGrb10tm1.1Fuis
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolGrb10tm1.1Fuis
Gene/Transgene symbolGrb10

Information from provider

ProviderFumitoshi Ishino
Provider affiliationTokyo Medical and Dental University, Medical Research Institute
Genetic informationDeletion of Meg1/Grb10 differentially methylated region (DMR).
Phenotypic informationHomozygous:
Small body size, black coat. NB: Grb10 gene is imprinted, so homozygosity is not required for mutant phenotype.

Heterozygous:
Small body size, black coat after paternal transmission of the mutation. Normal body size, black coat after maternal transmission of the mutation.
Breeding history1. Gene targeted CCE cells were injected into C57BL/6J blastocysts. 2. Male chimera was crossed with C57BL/6J female to create Grb10deltaDMRneo. 3. Grb10deltaDMRneo male was crossed with C57BL/6J female. 4. Fertilized Grb10deltaDMRneo heterozygous egg was injected with cre recombinase to create Grb10deltaDMR. 5. Grb10deltaDMR offspring were maintained in a heterozygous state by crossing with C57BL/6J. The number of generations is not known.
References
  • Paternal deletion of Meg1/Grb10 DMR causes maternalization of the Meg1/Grb10 cluster in mouse proximal Chromosome 11 leading to severe pre- and postnatal growth retardation.;Shiura Hirosuke, Nakamura Kenji, Hikichi Takafusa, Hino Toshiaki, Oda Kanako, Suzuki-Migishima Rika, Kohda Takashi, Kaneko-ishino Tomoko, Ishino Fumitoshi, ;2009;Human molecular genetics;18;1424-38; 19174477
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)
  • enlarged liver / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • abnormal pulmonary alveolus morphology / MGI
  • abnormal trachea morphology / MGI
  • increased insulin sensitivity / MGI
  • paternal imprinting / MGI
  • increased muscle weight / MGI
  • increased lean body mass / MGI
  • embryonic growth retardation / MGI
  • increased fetal size / MGI
  • decreased fetal size / MGI
  • small placenta / MGI
  • enlarged placenta / MGI
  • decreased placenta weight / MGI
  • growth/size/body region phenotype / MGI
  • increased lung weight / MGI
  • increased pancreas weight / MGI
  • increased birth body size / MGI
  • increased liver glycogen level / MGI
  • perinatal lethality, incomplete penetrance / MGI

Literature references

  • Paternal deletion of Meg1/Grb10 DMR causes maternalization of the Meg1/Grb10 cluster in mouse proximal Chromosome 11 leading to severe pre- and postnatal growth retardation.;Shiura Hirosuke, Nakamura Kenji, Hikichi Takafusa, Hino Toshiaki, Oda Kanako, Suzuki-Migishima Rika, Kohda Takashi, Kaneko-ishino Tomoko, Ishino Fumitoshi, ;2009;Human molecular genetics;18;1424-38; 19174477

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