C57BL/6N-Cdk4^{tm1c(NCOM)Mfgc}/TcpBiat

Status	Available to order
EMMA ID	EM:13809
Citation information	RRID:IMSR_EM:13809 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Cdk4^{tm1c(NCOM)Mfgc}/TcpBiat
Alternative name	C57BL/6N-Cdk4 tm1c(NCOM)Mfgc/Tcp
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Cdk4^{tm1c(NCOM)Mfgc}
Gene/Transgene symbol	Cdk4

Information from provider

Provider	Veronika Sexl
Provider affiliation	Department of Biomedical Sciences, University of Veterinary Medicine
Genetic information	The mouse line C57BL/6N-Cdk4^{tm1c(NCOM)Mfgc}/Tcp (referred to as Cdk4^fl/fl; MGI:6316287 allele ID) was generated as part of the NorCOMM2 project with C57BL/6N-Cdk4^{tm1a(NCOM)Mfgc}/Tcp (available from the Canadian Mouse Mutant Repository-CMMR at http://www.cmmr.ca/order.php?t=m&id=708) made from NorCOMM ES cells (Bradley A, Anastassiadis K, Ayadi A, et al. The mammalian gene function resource: the International Knockout Mouse Consortium. Mamm Genome. 2012;23:580-586) at the Toronto Centre for Phenogenomics, Canada. The strain is also available at CMMR at http://www.cmmr.ca/order.php?t=m&id=811
Phenotypic information	Homozygous: no data available; no phenotype expected. Heterozygous: no data available; no phenotype expected.
Breeding history	The flp recombinase-mediated excision was obtained by breeding a heterozygous C57BL/6N-Cdk4^{tm1a(NCOM)Mfgc}/Tcp male with heterozygous C57BL/6N-Gt(ROSA)26Sor^{tm2(CAG-flpo,-EYFP)Ics}/Tcp female(s). The mouse line was established by backcrossing Cdk4^tm1c+/-; flp^- mice to C57BL/6NCrl (less then 10 generations).
References	Inducible deletion of CDK4 and CDK6 - deciphering CDK4/6 inhibitor effects in the hematopoietic system.;Maurer Barbara, Brandstoetter Tania, Kollmann Sebastian, Sexl Veronika, Prchal-Murphy Michaela, ;2021;Haematologica;106;2624-2632; 32855282
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

IMPC phenotypes (gene matching)

hypoplasia / IMPC
abnormal embryo size / IMPC
preweaning lethality, incomplete penetrance / IMPC
developmental dysplasia / IMPC
decreased body length / IMPC
edema / IMPC
female infertility / IMPC
microphthalmia / IMPC
male infertility / IMPC
increased mean corpuscular volume / IMPC
abnormal cranium morphology / IMPC

MGI phenotypes (gene matching)

abnormal female reproductive system morphology / MGI
small ovary / MGI
abnormal male reproductive system morphology / MGI
abnormal testis morphology / MGI
small testis / MGI
Leydig cell hyperplasia / MGI
small seminiferous tubules / MGI
increased body weight / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal locomotor behavior / MGI
hyperactivity / MGI
polydipsia / MGI
impaired limb coordination / MGI
hyperglycemia / MGI
increased urine glucose level / MGI
polyuria / MGI
reduced male fertility / MGI
male infertility / MGI
female infertility / MGI
abnormal glucose homeostasis / MGI
postnatal lethality / MGI
abnormal fertility/fecundity / MGI
no abnormal phenotype detected / MGI
abnormal seminiferous tubule morphology / MGI
abnormal corpus luteum morphology / MGI
oligozoospermia / MGI
decreased circulating insulin level / MGI
abnormal Sertoli cell morphology / MGI
absent Leydig cells / MGI
decreased circulating follicle stimulating hormone level / MGI
ketoaciduria / MGI
abnormal cell cycle / MGI
impaired luteinization / MGI
increased pancreatic beta cell number / MGI
abnormal male reproductive system physiology / MGI
abnormal female reproductive system physiology / MGI
decreased testis weight / MGI
azoospermia / MGI
decreased circulating progesterone level / MGI
abnormal pancreatic islet morphology / MGI
homeostasis/metabolism phenotype / MGI
Leydig cell hypoplasia / MGI
decreased lactotroph cell number / MGI
adenohypophysis hypoplasia / MGI
delayed estrous cycle / MGI
small pancreatic islets / MGI
postnatal lethality, incomplete penetrance / MGI

Literature references

Inducible deletion of CDK4 and CDK6 - deciphering CDK4/6 inhibitor effects in the hematopoietic system.;Maurer Barbara, Brandstoetter Tania, Kollmann Sebastian, Sexl Veronika, Prchal-Murphy Michaela, ;2021;Haematologica;106;2624-2632; 32855282

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Cdk4tm1c(NCOM)Mfgc/TcpBiat