| Status | Available to order |
| EMMA ID | EM:13830 |
| Citation information | RRID:IMSR_EM:13830 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N;129-Mmachctm1.1Pg/Ieg |
| Alternative name | Mmachc flox/flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Mmachctm1.1Pg |
| Gene/Transgene symbol | Mmachc |
| Provider | Sean Froese |
| Provider affiliation | Division of Metabolism, University Children |
| Genetic information | Mmachc (references: NCBI gene ID:67096, MGI gene ID:1914346) is located on mouse chromosome 4, covers approximately 6 kilobases and contains 4 exons coding for 279 aa of the MMACHC protein. The translation start codon (ATG) is located in exon 1 and the translational stop site (TGA) in exon 4. To generate the Mmachc-flox/flox mice, a cassette of foreign DNA containing loxP-FRT-neomycin-FRT sequences was inserted into intron 2, 143 bp upstream of exon 3. The second loxP site was inserted into the 3’UTR of exon 4, 220 bp downstream of the STOP codon. After flp-mediated recombination to delete the neomycin gene, a single FRT site remained in intron 2. |
| Phenotypic information | Homozygous:There is no phenotype without cross-breeding to cre recombinase-expressing mice. The phenotype of the resulting matings depends on the cre recombinase promotor.Heterozygous:No phenotype. |
| Breeding history | The mice were kept in the homozygous Mmachc-flox/flox state by always breeding Mmachc-flox/flox mice with other Mmachc-flox/flox mice from the same line. More than 20 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6N males |
Orphanet associated rare diseases, based on orthologous gene matching
