IMPC phenotypes (gene matching)
B6;129P2-Rgs4tm1Jbr/Orl
| Status | Available to order |
| EMMA ID | EM:01385 |
| Citation information | RRID:IMSR_EM:01385 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Rgs4tm1Jbr/Orl |
| Alternative name | Rgs4loxFRTlacZ |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Rgs4tm1Jbr |
| Gene/Transgene symbol | Rgs4 |
Information from provider
| Provider | Jean-François Brunet |
| Provider affiliation | CNRS/ Ecole normale supérieure |
| Genetic information | The targeting vector was derived from a 129/Ola cosmid encompassing the Rgs4 locus (RZPD, Berlin) and consisted of an 11 kb 5'-homology arm and a 1.12 kb 3'-homology arm separated by an FRT-flanked neo cassette, itself preceded by a loxP/splice-acceptor site/promoter-less lacZ cassette; the neo and lacZ cassettes replaced a 1.2 kb region located 0.6 kb downstream of the Rgs4 polyA signal. Upstream (4.5 kb) of these two cassettes, a second loxP site and a third FRT site were introduced in the second intron of Rgs4, so that recombination by the Flp or cre recombinase would interrupt the Rgs4 coding sequences before the RGS domain. Recombination by cre inactivates Rgs4 and switches on lacZ, recombination by Flp either deletes the neo gene, or inactivates Rgs4 without switching on lacZ. |
| Phenotypic information | None. |
| Breeding history | One backcross to C57BL/6. |
| References |
|
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Disease and phenotype information
MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
MGI phenotypes (gene matching)
- decreased body weight / MGI
- hyperactivity / MGI
- impaired coordination / MGI
- increased circulating HDL cholesterol level / MGI
- abnormal touch/ nociception / MGI
- no abnormal phenotype detected / MGI
- increased vertical activity / MGI
- no phenotypic analysis / MGI
- nervous system phenotype / MGI
- decreased aggression towards mice / MGI
- increased circulating cholesterol level / MGI
- behavior/neurological phenotype / MGI
- decreased renal plasma flow rate / MGI
- impaired conditioned place preference behavior / MGI
- impaired behavioral response to morphine / MGI
- increased sensitivity to xenobiotic induced morbidity/mortality / MGI
- decreased behavioral withdrawal response / MGI
- increased total body fat amount / MGI
- embryonic lethality between somite formation and embryo turning, incomplete penetrance / MGI
Literature references
- Association and linkage analyses of RGS4 polymorphisms in schizophrenia.;Chowdari Kodavali V, Mirnics Karoly, Semwal Prachi, Wood Joel, Lawrence Elizabeth, Bhatia Triptish, Deshpande Smita N, B K Thelma, Ferrell Robert E, Middleton Frank A, Devlin Bernie, Levitt Pat, Lewis David A, Nimgaonkar Vishwajit L, ;2002;Human molecular genetics;11;1373-80; 12023979
- RGS4 causes increased mortality and reduced cardiac hypertrophy in response to pressure overload.;Rogers J H, Tamirisa P, Kovacs A, Weinheimer C, Courtois M, Blumer K J, Kelly D P, Muslin A J, ;1999;The Journal of clinical investigation;104;567-76; 10487771
- Generation and characterization of Rgs4 mutant mice.;Grillet Nicolas, Pattyn Alexandre, Contet Candice, Kieffer Brigitte L, Goridis Christo, Brunet Jean-François, ;2005;Molecular and cellular biology;25;4221-8; 15870291