| Status | Available to order |
| EMMA ID | EM:01417 |
| Citation information | RRID:IMSR_EM:01417 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Prkaa1tm1Sbj/Orl |
| Alternative name | AMPKalpha1-knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Prkaa1tm1Sbj |
| Gene/Transgene symbol | Prkaa1 |
| Provider | Sophie Vaulont |
| Provider affiliation | INSERMU567, CNRS UMR8401, universite Paris 5, Institut Cochin |
| Genetic information | A mouse 129-strain genomic library (Stratagene) was screened with a specific mouse AMPK catalytic alpha1-subunit (Prkaa1) 500-bp fragment. One genomic clone encompassing a 14.5-kb genomic fragment was used to generate the targeting construct, which was linearized at an unique XhoI site and used to electroporate embryonic stem (ES) cells (gift from Anne K. Voss, Gottingen), which were cultured on mitomycin-treated embryonic fibroblast feeder layers. DNA from G418-selected clones was analyzed on Southern blot. DNA was EcoRI-digested and hybridized either with a 5'-external probe consisting of a genomic SalI-HincII fragment or with a 3'-external probe consisting of a SacI-SacI genomic fragment. Positive ES cells were injected into blastocysts derived from C57BL/6J mice, and chimeric males were mated to wild-type C57BL/6J females for germ line transmission. |
| Phenotypic information | Homozygous embryos die in utero on C57BL/6 background but not on a B6 x 129 background. On mixed background, no defect in glucose homoeostasis was observed in AMPK alpha 1 knock-out mice but the weight of different adipose tissue depots is strongly reduced in these mice, suggesting the potential anti-lipolytic role of AMPK in this tissue. |
| Breeding history | AMPKalpha1(+/-) mice were backcrossed 10 times to C57BL/6J background at the CDTA germ-free animal house in Orleans. At this point no homozygous animals were obtained so it was decided to breed the line with heterozygous animals. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
