B6.129-Prkaa2^tm1Vio/Orl

Status	Available to order
EMMA ID	EM:01420
Citation information	RRID:IMSR_EM:01420 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129-Prkaa2^tm1Vio/Orl
Alternative name	AMPKalpha2_floxed
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Prkaa2^tm1Vio
Gene/Transgene symbol	Prkaa2

Information from provider

Provider	Sophie Vaulont
Provider affiliation	INSERMU567, CNRS UMR8401, universite Paris 5, Institut Cochin
Genetic information	Protein kinase, AMP-activated, alpha 2 (Prkaa2 or AMPK alpha2) locus. A mouse 129 strain lambda genomic library (Stratagene) was screened with a AMPK alpha2 500-bp fragment, made by reverse-transcription polymerase chain reaction on liver mRNA. This fragment contains the exon encoding the C-terminus part of AMPK alpha2 catalytic domain (exon C9). To introduce a loxP site, a double-stranded oligonucleotide (C-loxP-HindIII-SphI), was cloned 300 bp upstream of exon C. Embryonic stem cells (MPI) were electroporated with the linearized targeting construct and selected; clones heterozygous for the floxed allele were injected into C57BL/6 blastocysts. Chimeric male mice were crossed with C57BL/6 female mice to generate heterozygous floxed/wild-type allele mice, and heterozygous mice were mated to generate homozygous mice.
Phenotypic information	No apparent defect.
Breeding history	AMPKalpha2_floxed(+/-) mice were backcrossed 6 times to C57BL/6J background at the CDTA germ-free animal house in Orleans.
References	The AMP-activated protein kinase alpha2 catalytic subunit controls whole-body insulin sensitivity.;Viollet Benoit, Andreelli Fabrizio, Jørgensen Sebastian B, Perrin Christophe, Geloen Alain, Flamez Daisy, Mu James, Lenzner Claudia, Baud Olivier, Bennoun Myriam, Gomas Emmanuel, Nicolas Gaël, Wojtaszewski Jørgen F P, Kahn Axel, Carling David, Schuit Frans C, Birnbaum Morris J, Richter Erik A, Burcelin Rémy, Vaulont Sophie, ;2003;The Journal of clinical investigation;111;91-8; 12511592 Cre-mediated germline mosaicism: a method allowing rapid generation of several alleles of a target gene.;Holzenberger M, Lenzner C, Leneuve P, Zaoui R, Hamard G, Vaulont S, Bouc Y L, ;2000;Nucleic acids research;28;E92; 11058142 A tamoxifen-inducible chimeric Cre recombinase specifically effective in the fetal and adult mouse liver.;Tannour-Louet Mounia, Porteu Arlette, Vaulont Sophie, Kahn Axel, Vasseur-Cognet Mireille, ;2002;Hepatology (Baltimore, Md.);35;1072-81; 11981757

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France

Disease and phenotype information

IMPC phenotypes (gene matching)

increased circulating alanine transaminase level / IMPC
enlarged spleen / IMPC
increased circulating creatine kinase level / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased circulating serum albumin level / IMPC
decreased circulating fructosamine level / IMPC
increased circulating aspartate transaminase level / IMPC
decreased fasting circulating glucose level / IMPC
decreased exploration in new environment / IMPC

MGI phenotypes (gene matching)

increased circulating free fatty acid level / MGI
hyperglycemia / MGI
abnormal glucose homeostasis / MGI
decreased circulating insulin level / MGI
decreased insulin secretion / MGI
abnormal muscle cell glucose uptake / MGI
abnormal sympathetic nervous system physiology / MGI
impaired glucose tolerance / MGI
insulin resistance / MGI
homeostasis/metabolism phenotype / MGI
abnormal glycogen homeostasis / MGI
decreased glycogen level / MGI
decreased circulating leptin level / MGI
abnormal urine catecholamine level / MGI

Literature references

The AMP-activated protein kinase alpha2 catalytic subunit controls whole-body insulin sensitivity.;Viollet Benoit, Andreelli Fabrizio, Jørgensen Sebastian B, Perrin Christophe, Geloen Alain, Flamez Daisy, Mu James, Lenzner Claudia, Baud Olivier, Bennoun Myriam, Gomas Emmanuel, Nicolas Gaël, Wojtaszewski Jørgen F P, Kahn Axel, Carling David, Schuit Frans C, Birnbaum Morris J, Richter Erik A, Burcelin Rémy, Vaulont Sophie, ;2003;The Journal of clinical investigation;111;91-8; 12511592
Cre-mediated germline mosaicism: a method allowing rapid generation of several alleles of a target gene.;Holzenberger M, Lenzner C, Leneuve P, Zaoui R, Hamard G, Vaulont S, Bouc Y L, ;2000;Nucleic acids research;28;E92; 11058142
A tamoxifen-inducible chimeric Cre recombinase specifically effective in the fetal and adult mouse liver.;Tannour-Louet Mounia, Porteu Arlette, Vaulont Sophie, Kahn Axel, Vasseur-Cognet Mireille, ;2002;Hepatology (Baltimore, Md.);35;1072-81; 11981757
Endothelial AMP-Activated Kinase α1 Phosphorylates eNOS on Thr495 and Decreases Endothelial NO Formation.;Zippel Nina, Loot Annemarieke E, Stingl Heike, Randriamboavonjy Voahanginirina, Fleming Ingrid, Fisslthaler Beate, ;2018;International journal of molecular sciences;19;; 30217073

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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Practical information

Genotyping protocol

Example health report
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B6.129-Prkaa2tm1Vio/Orl