CD1;B6N-Igs40tm2(ACTB-tdTomato,-EGFP)Biat/Biat
| Status | Available to order |
| EMMA ID | EM:14721 |
| Citation information | RRID:IMSR_EM:14721 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | CD1;B6N-Igs40tm2(ACTB-tdTomato,-EGFP)Biat/Biat |
| Alternative name | CD1;B6N-MADM-TGtm1(Chr19)Biat; Igs40tm2(ACTB-tdTomato,-EGFP)Biat; MADM-19-TG |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Igs40tm2(ACTB-tdTomato,-EGFP)Biat |
| Gene/Transgene symbol | Igs40 |
Information from provider
| Provider | Simon Hippenmeyer |
| Provider affiliation | Institute of Science and Technology Austria (IST Austria) |
| Genetic information | The TG mosaic analysis with double markers (MADM) targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the Igs40 (Hipp19) locus on chromosome 19 (between Rela and Sipa1; PMID 34161767). |
| Phenotypic information | Homozygous:not availableHeterozygous:not available |
| Breeding history | Chimeras were generated by injecting C57BL/6N embryonic stem cells into BALB/cRj blastocysts and then crossed with CD1 mice. Afterwards they were intercrossed. |
| References |
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| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- A genome-wide library of MADM mice for single-cell genetic mosaic analysis.;Contreras Ximena, Amberg Nicole, Davaatseren Amarbayasgalan, Hansen Andi H, Sonntag Johanna, Andersen Lill, Bernthaler Tina, Streicher Carmen, Heger Anna, Johnson Randy L, Schwarz Lindsay A, Luo Liqun, Rülicke Thomas, Hippenmeyer Simon, ;2021;Cell reports;35;109274; 34161767
- Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967
- Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859
- Protocol for sorting cells from mouse brains labeled with mosaic analysis with double markers by flow cytometry.;Amberg Nicole, Cheung Giselle, Hippenmeyer Simon, ;2024;STAR protocols;5;102771; 38070137
- Multipotent progenitors instruct ontogeny of the superior colliculus.;Cheung Giselle, Pauler Florian M, Koppensteiner Peter, Krausgruber Thomas, Streicher Carmen, Schrammel Martin, Gutmann-Özgen Natalie, Ivec Alexis E, Bock Christoph, Shigemoto Ryuichi, Hippenmeyer Simon, ;2024;Neuron;112;230-246.e11; 38096816
- Critical role of cell competition in gliomagenesis.;Jiang Ying, Ahn Ryuhjin, Huang Arthur, Gonzalez Phillippe P, Kim Jungeun, Zhang Guoxin, Liu Zihao, He Zhenqiang, Dudley Lindsey, Patel Kunal S, Dzhivhuho Godfrey A, Crowl Sam, Przanowski Piotr, Camacho Luisa Quesada, Hao Sijie, Zeng Jianhao, Hippenmeyer Simon, Fallahi-Sichani Mohammad, Janes Kevin A, Naegle Kristen M, Hammarskjold Marie-Louise, Goldman Steven A, Kornblum Harley I, Yao Maojin, White Forest, Zong Hui, ;2026;bioRxiv : the preprint server for biology;0;; 41648527