IMPC phenotypes (gene matching)
B6(Cg)-Rps6ka1Cys421Ser/Cys424Ser/H
| Status | Available to order |
| EMMA ID | EM:14900 |
| Citation information | RRID:IMSR_EM:14900 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6(Cg)-Rps6ka1Cys421Ser/Cys424Ser/H |
| Alternative name | Cys421Ser/Cys424Ser RSK1 |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Rps6ka1Cys421Ser/Cys424Ser |
| Gene/Transgene symbol | Rps6ka1 |
Information from provider
| Provider | Philip Eaton |
| Provider affiliation | Clinical Pharmacology, QMUL |
| Genetic information | Constitutive global C421S and C424S mutations knock-in in the mouse Rps6ka1 (Rsk1) gene. The Rps6ka1 gene (GenBank accession number: NM_009097.4, Ensembl: ENSMUSG00000003644) is located on mouse chromosome 4; 22 exons have been identified, with the ATG start codon in exon 1 and TGA stop codon in exon 22; the amino acid residues C421 and C424 are located in exon 15. To engineer the targeting vector, 5’ homology arm and 3’ homology arm were amplified from BAC DNA and confirmed by end sequencing. The C421S mutation (TGT to TCT) and C424S mutation (TGT to TCT) were introduced into exon 15 in 3’ homology arm. In the targeting vector, the neo cassette was flanked by loxP sites. DTA was used for negative selection. The constitutive knock-in allele was obtained after cre-mediated recombination. C57BL/6 ES cells were used for gene targeting. |
| Phenotypic information | Homozygous:No harmful phenotype was recorded so farHeterozygous:No harmful phenotype was recorded so far |
| Breeding history | Mice were back-crossed to C57BL/6J for minimum 10 generations. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |