IMPC phenotypes (gene matching)
B6;129P2-Hprt1em1(RNU6-gRNA:Top1,-mCherry)Jtu/H
| Status | Available to order |
| EMMA ID | EM:15010 |
| Citation information | RRID:IMSR_EM:15010 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Hprt1em1(RNU6-gRNA:Top1,-mCherry)Jtu/H |
| Alternative name | Hprttm1(CAG-mCherry Top1)Jtu |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Hprt1tm1(CAG-mCherry-Top1)Jtu |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | James Turner |
| Provider affiliation | Sex Chromosome Biology Laboratory, The Francis Crick Institute |
| Additional owner | Genoway, FRANCE |
| Genetic information | The mice harbour an sgRNA transgene targeting Top1 gene which has been inserted into Hprt1 locus. The Top1 sgRNA targeting vector was generated using plasmid pX333 as a backbone (Addgene). The Cas9 cassette was replaced with an mCherry reporter. Top1 sgRNA2 were inserted using BbsI. The resulting hU6-sgRNA2-pCbh-mCherry reporter construct was used to generate the XTop1 transgene by GenOway (Lyon, France) using their Quick Knock-in approach in E14Tg2a mESCs derived from 129P2/OlaHsd. Human HPRT1 exons 1 and 2 were inserted into the sgRNA2-mCherry targeting vector. The construct was targeted to the Hprt1 locus in mESCs that lacked exons 1 and 2. Upon recombination of the targeting vector into Hprt1, gene function was restored. Correctly targeted clones were selected with hypoxanthine-aminopterin-thymidine (HAT) medium. Eight HAT-resistant clones were selected and amplified for confirmation of on-target integration. X-integration clones were confirmed using PCR and Sanger sequencing for validation. |
| Phenotypic information | Homozygous:Unknown. Homozygous mice were not generated for our experiments.Heterozygous:Viable and fertile. |
| Breeding history | 4 generations of backcrosses to C57BL/6 |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- increased neutrophil cell number / MGI
- abnormal small intestine morphology / MGI
- abnormal liver morphology / MGI
- abnormal branching of the mammary ductal tree / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- enlarged lymph nodes / MGI
- tremors / MGI
- convulsive seizures / MGI
- abnormal lung morphology / MGI
- decreased body weight / MGI
- decreased anxiety-related response / MGI
- ataxia / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- short stride length / MGI
- decreased exploration in new environment / MGI
- limb grasping / MGI
- abnormal motor coordination/balance / MGI
- abnormal hematopoietic system physiology / MGI
- hyperglycemia / MGI
- anemia / MGI
- cardiac hypertrophy / MGI
- increased mammary adenocarcinoma incidence / MGI
- abnormal reflex / MGI
- seizures / MGI
- abnormal motor capabilities/coordination/movement / MGI
- premature death / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal brain morphology / MGI
- no abnormal phenotype detected / MGI
- neurodegeneration / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- hepatic steatosis / MGI
- decreased vertical activity / MGI
- increased heart weight / MGI
- increased systemic arterial blood pressure / MGI
- albuminuria / MGI
- decreased erythrocyte cell number / MGI
- increased urine protein level / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- phenotypic reversion / MGI
- abnormal dopaminergic neuron morphology / MGI
- astrocytosis / MGI
- abnormal depression-related behavior / MGI
- decreased tumor growth/size / MGI
- abnormal nervous system morphology / MGI
- abnormal cardiac muscle relaxation / MGI
- neuronal intranuclear inclusions / MGI
- abnormal myocardial fiber physiology / MGI
- abnormal Paneth cell morphology / MGI
- decreased B cell number / MGI
- decreased cardiac muscle contractility / MGI
- glomerulosclerosis / MGI
- abnormal podocyte morphology / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- behavior/neurological phenotype / MGI
- immune system phenotype / MGI
- taste/olfaction phenotype / MGI
- hematopoietic system phenotype / MGI
- jerky movement / MGI
- thrombocytosis / MGI
- decreased ventricle muscle contractility / MGI
- decreased mean corpuscular hemoglobin concentration / MGI
- decreased dopamine level / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- absent podocyte slit diaphragm / MGI
- podocyte foot process effacement / MGI
- increased megakaryocyte cell number / MGI
- abnormal spatial reference memory / MGI
- abnormal spatial working memory / MGI
- abnormal splenic cell ratio / MGI
- abnormal physiological response to xenobiotic / MGI
- abnormal enterocyte proliferation / MGI
- abnormal enterocyte apoptosis / MGI
- abnormal neuron differentiation / MGI
- increased mammary gland tumor incidence / MGI
- myeloid hyperplasia / MGI
- expanded mesangial matrix / MGI
- mesangial cell hyperplasia / MGI
- abnormal habituation to a new environment / MGI
- abnormal ceramide level / MGI
- decreased brain choline acetyltransferase activity / MGI
- decreased brain tyrosine 3-monooxygenase activity / MGI
- decreased vascular endothelial cell proliferation / MGI
Literature references
- CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes.;Douglas Charlotte, Maciulyte Valdone, Zohren Jasmin, Snell Daniel M, Mahadevaiah Shantha K, Ojarikre Obah A, Ellis Peter J I, Turner James M A, ;2021;Nature communications;12;6926; 34862376