B6JHsd.129-Gfra4tm1Msa/Oulu
| Status | Available to order |
| EMMA ID | EM:15022 |
| Citation information | RRID:IMSR_EM:15022 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6JHsd.129-Gfra4tm1Msa/Oulu |
| Alternative name | Gfra4KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gfra4tm1Msa |
| Gene/Transgene symbol | Gfra4 |
Information from provider
| Provider | Matti Airaksinen |
| Provider affiliation | Univ.Helsinki |
| Genetic information | Targeted deletion of the Gfra4 gene. A 1440-bp KpnI-SacII fragment of the Gfra4 gene containing exons II–V was replaced with a 1.6-kb PGKneo cassette. |
| Phenotypic information | Homozygous:No differences were observed between wild-type and Gfra4-KO littermate animals in growth, gross behaviour, or viability. The number and morphology of the thyroid C cells were indistinguishable between the genotypes in both newborn and adult age. However, thyroid tissue calcitonin content was reduced by 60% in newborn and by 45% in 3 weeks-old Gfra4-KO mice compared with wild-type controls. Consistent with the reduced calcitonin levels, bone formation rate in juvenile Gfra4-KO mice was increased.Heterozygous:Not tested. |
| Breeding history | The mutant Gfra4 allele was transferred into C57BL/6JOlaHsd mouse background by backcrossing for at least five generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
Literature references
- Ablation of persephin receptor glial cell line-derived neurotrophic factor family receptor alpha4 impairs thyroid calcitonin production in young mice.;Lindfors Päivi H, Lindahl Maria, Rossi Jari, Saarma Mart, Airaksinen Matti S, ;2006;Endocrinology;147;2237-44; 16497798