C57BL/6-Tn(Nr4a1-mCherry*)M4Cya/H
| Status | Available to order |
| EMMA ID | EM:15078 |
| Citation information | RRID:IMSR_EM:15078 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tn(Nr4a1-mCherry*)M4Cya/H |
| Alternative name | Nur77-Tempo |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tn(Nr4a1-mCherry*)M4Cya |
| Gene/Transgene symbol | Tn(Nr4a1-mCherry*)M4Cya |
Information from provider
| Provider | David Bending |
| Provider affiliation | University of Birmingham |
| Genetic information | Taconic/Cyagen were contracted to design and produce the transgenic mouse. Bacterial artificial chromosome (BAC) RP24-366J14 containing the mouse Nr4a1 (Nur77) gene and regulatory regions was used as the starting template. A Fast-FT-rBG-pA-zeomycin selection cassette was inserted into exon 2 (first coding exon) of Nr4a1 via homologous recombination (FT: Fluorescent Timer protein-coding sequence). The zeomycin cassette was subsequently removed by homologous combination. PiggyBac ITR-flanked ampicillin selection cassette was introduced into the BAC vector backbone and the SacBII and loxP sites were replaced. The two ITR elements are recognition sequences of the piggyBac transposase (PBase). Amp expression cassettes were used for selection of correctly modified BACs. The modified BAC was injected into C57BL/6J single-cell stage fertilized eggs. The region from 5’ ITR to 3’ ITR on the BAC (which contains the Nr4a1-FT-Fast transgene) was transposed into random TTAA sites of the host genome mediated by PBase, with one integration copy per site. Pups were then genotyped for the presence of the modified BAC. |
| Phenotypic information | Homozygous:The mutation does not result in any overt phenotype. Mice will express the FT-Fast (Fluorescent Timer-Fast) protein in cells that activate Nr4a1 expression.Heterozygous:The mutation does not result in any overt phenotype. Mice will express the FT-Fast protein in cells that activate Nr4a1 expression. |
| Breeding history | 8 founder lines were established which were mated to C57BL/6 mice. 4 founder lines achieved germline transmission (M2, M3, M4 and F2) of the modified BAC. Founders were bred to achieve single copy transmission and phenotype was confirmed by flow cytometry for expression of the FT-Fast transgene. Lines M4 and F2 were chosen for further expansion and mated Tg x wt or Tg x Tg to achieve homozygous BAC transgenics. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Nur77-Tempo mice reveal T cell steady state antigen recognition.;Elliot Thomas A E, Jennings Emma K, Lecky David A J, Rouvray Sophie, Mackie Gillian M, Scarfe Lisa, Sheriff Lozan, Ono Masahiro, Maslowski Kendle M, Bending David, ;2022;Discovery immunology;1;kyac009; 36704407
- Salmonella cancer therapy metabolically disrupts tumours at the collateral cost of T cell immunity.;Copland Alastair, Mackie Gillian M, Scarfe Lisa, Jinks Elizabeth, Lecky David A J, Gudgeon Nancy, McQuade Riahne, Ono Masahiro, Barthel Manja, Hardt Wolf-Dietrich, Ohno Hiroshi, Hoevenaar Wilma H M, Dimeloe Sarah, Bending David, Maslowski Kendle M, ;2024;EMBO molecular medicine;16;3057-3088; 39558103