IMPC phenotypes (gene matching)
- preweaning lethality, complete penetrance / IMPC
129.129P2-Map2k1tm1Bacc/Biat
| Status | Available to order |
| EMMA ID | EM:15085 |
| Citation information | RRID:IMSR_EM:15085 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129.129P2-Map2k1tm1Bacc/Biat |
| Alternative name | 129Sv Mek1 F/F |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Map2k1tm1Bacc |
| Gene/Transgene symbol | Map2k1 |
Information from provider
| Provider | Manuela Baccarini |
| Provider affiliation | University of Vienna |
| Genetic information | A targeting vector containing loxP sites 5′ and 3′ of exon 2 was designed. A third loxP site and a selection cassette (a neomycin resistance gene for positive selection and the HSV thymidine kinase gene for negative selection) were inserted upstream of the floxed exon 2. The mutation was introduced into E14.1 embryonic stem cells by homologous recombination, and the neo/tk gene cassette was then deleted by transiently expressing the cre recombinase. See Catalanotti F, et al., A Mek1-Mek2 heterodimer determines the strength and duration of the Erk signal, Nat Struct Mol Biol. 2009, Mar;16(3):294-303. |
| Phenotypic information | Homozygous:Map2k1 (Mek1) (f/f) mice are phenotypically normal and fertile. When bred to mice expressing the cre recombinase under the control of a tissue-specific promoter, exon 2 of Mek1 is deleted, which leads to the complete loss of the MEK1 protein in the tissue of interest.Heterozygous:Mice are phenotypically normal and fertile |
| Breeding history | Backcrossed to 129/Sv background for more than 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous 129 males |
Disease and phenotype information
Literature references
- A Mek1-Mek2 heterodimer determines the strength and duration of the Erk signal.;Catalanotti Federica, Reyes Gloria, Jesenberger Veronika, Galabova-Kovacs Gergana, de Matos Simoes Ricardo, Carugo Oliviero, Baccarini Manuela, ;2009;Nature structural & molecular biology;16;294-303; 19219045
- MEK1 is required for PTEN membrane recruitment, AKT regulation, and the maintenance of peripheral tolerance.;Zmajkovicova Katarina, Jesenberger Veronika, Catalanotti Federica, Baumgartner Christian, Reyes Gloria, Baccarini Manuela, ;2013;Molecular cell;50;43-55; 23453810
- An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion.;Baumgartner Christian, Toifl Stefanie, Farlik Matthias, Halbritter Florian, Scheicher Ruth, Fischer Irmgard, Sexl Veronika, Bock Christoph, Baccarini Manuela, ;2018;Cell stem cell;22;879-892.e6; 29804890