B6.129P2-Vti1b^tm1Gfvm Vti1a^tm1Gfvm/Ieg

Status	Available to order
EMMA ID	EM:15088
Citation information	RRID:IMSR_EM:15088 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Vti1b^tm1Gfvm Vti1a^tm1Gfvm/Ieg
Alternative name	vti1a-/- vti1b-/-
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Vti1b^tm1Gfvm, Vti1a^tm1Gfvm
Gene/Transgene symbol	Vti1b, Vti1a

Information from provider

Provider	Gabriele Fischer von Mollard
Provider affiliation	Universität Bielefeld
Genetic information	A neomycin resistance cassette has been inserted into exon 6 of Vti1a encoding amino acid residues 115-142 by homologous recombination in ES cells. A neomycine resistant cassette has been inserted into exon 4 of Vti1b encoding amino acid residues 123-180 by homologous recombination in ES cells.
Phenotypic information	Homozygous: Vti1a-/- Vti1b-/- embryos die perinatally. At E18.5 their frequency is below expectation. They are smaller. Their neuronal development is impaired with defect in major axon tracts, neurodegeneration in several ganglia and malformation in cortical layers. Heterozygous: Vti1a+/- Vti1b+/- are indistinguishable from wild-type and serve as controls in our experiments.
Breeding history	The ES cell line E14.1 from 129Ola was used. Offspring was mated with C57BL/6 mice. Mouse strains were maintained with a mixed C57BL/6 x 129/Sv background for several years. Mice were backcrossed to C57BL/6 for 10 generations. Vti1a-/- mice were crossed with Vti1b-/- mice and are now bred as a single strain with Vti1a+/- Vti1b+/-, Vti1a-/- Vti1b+/- and Vti1a+/- Vti1b-/- mice. C57BL/6 is crossed in once or twice a year.
References	Lack of the endosomal SNAREs vti1a and vti1b led to significant impairments in neuronal development.;Kunwar Ajaya J, Rickmann Michael, Backofen Bianca, Browski Sascha M, Rosenbusch Joachim, Schöning Susanne, Fleischmann Thomas, Krieglstein Kerstin, Fischer von Mollard Gabriele, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;2575-80; 21262811 Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.;Emperador-Melero Javier, Huson Vincent, van Weering Jan, Bollmann Christian, Fischer von Mollard Gabriele, Toonen Ruud F, Verhage Matthijs, ;2018;Nature communications;9;3421; 30143604 Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development.;Bollmann Christian, Schöning Susanne, Kotschnew Katharina, Grosse Julia, Heitzig Nicole, Fischer von Mollard Gabriele, ;2022;Neural development;17;12; 36419086 Ablation of Vti1a/1b Triggers Neural Progenitor Pool Depletion and Cortical Layer 5 Malformation in Late-embryonic Mouse Cortex.;Sokpor Godwin, Rosenbusch Joachim, Kunwar Ajaya J, Rickmann Michael, Tuoc Tran, Rizzoli Silvio O, Tarabykin Victor, von Mollard Gabriele Fischer, Krieglstein Kerstin, Staiger Jochen F, ;2021;Neuroscience;463;303-316; 33774122 Alterations in bone malformation in the absence of the endosomal SNAREs Vti1a and Vti1b.;Schmücker Simone, Schöning Susanne, Wiegand Christiane, Höfling Sascha Michael, Bollmann Christian, Koliwer Judith, Fischer von Mollard Gabriele, ;2026;PloS one;21;e0343070; 41838692
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6JRj (Centre D'Elevage R. Janvier) males

Disease and phenotype information

IMPC phenotypes (gene matching)

increased circulating alkaline phosphatase level / IMPC
decreased thigmotaxis / IMPC
abnormal epididymis morphology / IMPC
abnormal behavior / IMPC
hyperactivity / IMPC
increased vertical activity / IMPC
enlarged epididymis / IMPC
abnormal testis morphology / IMPC
decreased body length / IMPC
abnormal auditory brainstem response / IMPC
limb grasping / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased fasting circulating glucose level / IMPC

Literature references

Lack of the endosomal SNAREs vti1a and vti1b led to significant impairments in neuronal development.;Kunwar Ajaya J, Rickmann Michael, Backofen Bianca, Browski Sascha M, Rosenbusch Joachim, Schöning Susanne, Fleischmann Thomas, Krieglstein Kerstin, Fischer von Mollard Gabriele, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;2575-80; 21262811
Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.;Emperador-Melero Javier, Huson Vincent, van Weering Jan, Bollmann Christian, Fischer von Mollard Gabriele, Toonen Ruud F, Verhage Matthijs, ;2018;Nature communications;9;3421; 30143604
Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development.;Bollmann Christian, Schöning Susanne, Kotschnew Katharina, Grosse Julia, Heitzig Nicole, Fischer von Mollard Gabriele, ;2022;Neural development;17;12; 36419086
Ablation of Vti1a/1b Triggers Neural Progenitor Pool Depletion and Cortical Layer 5 Malformation in Late-embryonic Mouse Cortex.;Sokpor Godwin, Rosenbusch Joachim, Kunwar Ajaya J, Rickmann Michael, Tuoc Tran, Rizzoli Silvio O, Tarabykin Victor, von Mollard Gabriele Fischer, Krieglstein Kerstin, Staiger Jochen F, ;2021;Neuroscience;463;303-316; 33774122
Alterations in bone malformation in the absence of the endosomal SNAREs Vti1a and Vti1b.;Schmücker Simone, Schöning Susanne, Wiegand Christiane, Höfling Sascha Michael, Bollmann Christian, Koliwer Judith, Fischer von Mollard Gabriele, ;2026;PloS one;21;e0343070; 41838692

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B6.129P2-Vti1btm1Gfvm Vti1atm1Gfvm/Ieg