| Status | Available to order |
| EMMA ID | EM:15141 |
| Citation information | RRID:IMSR_EM:15141 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tfrctm1(TFRC)Bdes/Orl |
| Alternative name | Tfrctm1(TFRC)Bdes |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Tfrctm1(TFRC)Bdes |
| Gene/Transgene symbol | Tfrc |
| Provider | Lutgarde Serneels |
| Provider affiliation | VIB-KU Leuven Center for Brain & Disease Research, VIB |
| Genetic information | For the knock-in model, the sequences of mouse Tfrc (aa. 196-381) were replaced with the sequences of human TFRC (aa. 194-379). As templates for the generation of the targeting vector, BAC RP24-363L9 and RP23-206N24 from the C57BL/6 library and RP11-480A16 were used. C57BL/6 ES cells (Cyagen) were used to target the Tfrc gene in C57BL/6 mice. This resulted in mice expressing a TFRC protein with a humanized apical domain (API). |
| Phenotypic information | Homozygous:No obvious phenotype is found. Mice are normal, viable, fertile, and produce normal sized litters.Heterozygous:Heterozygous pups are smaller and weaker compared to their wild-type and homozygous littermates. Heterozygous mice catch up the weight differences and appear viable and fertile. This phenotype is not studied in detail, but as TFRC acts as a dimer we believe it may be due to less functional dimers containing wild-type mouse TFRC and the humanized API_TFCR in the heterozygous animals. |
| Breeding history | This strain was generated by Cyagen and F1 heterozygous animals were crossed to generate homozygous offspring. The mice were kept for 8 generations as homozygous breeders. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6 males, homozygous C57BL/6 females |
| Stage of embryos | 2-cell |
Orphanet associated rare diseases, based on orthologous gene matching
