C57BL/6-Dnmt1tm(tetO)Cstw/H
| Status | Available to order |
| EMMA ID | EM:15188 |
| Citation information | RRID:IMSR_EM:15188 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Dnmt1tm(tetO)Cstw/H |
| Alternative name | C57BL/6 DNMT1-FAST |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Dnmt1tm1(FAST) |
| Gene/Transgene symbol | Dnmt1 |
Information from provider
| Provider | Christopher Switzer |
| Provider affiliation | Queen Mary University of London |
| Genetic information | F.A.S.T. (Flexible Accelerated STOP TetO) cassette consisting of a loxP-Frt-neo-Frt-STOP-Frt-PTRE3G-loxP sequence and Not I cloning site, knocked into the Dnmt1 gene locus. A 9.6 kb region was subcloned from a positively identified C57BL/6 fosmid clone (WI1-560618) and used to assemble a targeting vector carrying the F.A.S.T. cassette, consisting of a loxP-Frt-neo-Frt-STOP-Frt-PTRE3G-loxP sequence and a Not I cloning site. A Not I site downstream of the exon 1 of Dnmt1 was modified to allow the cloning of the F.A.S.T. knock-in cassette. The short homology arm (SA) of 2.8 kb and the long homology arm (LA) of 6.7kb enable the homologous recombination. The targeting vector was confirmed by restriction analysis and sequencing after each modification step. The boundaries of the vector were confirmed by sequencing with primers P6 and T73. The F.A.S.T. cassette sequence was confirmed by sequencing with primers SQ1, P2, and FAST SQ1. The point mutation to removes the Not I site was confirmed by FAST SQ1. |
| Phenotypic information | Homozygous:No observable phenotypic differences.Heterozygous:No observable phenotypic differences. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autosomal dominant cerebellar ataxia-deafness-narcolepsy syndrome / Orphanet_314404
- Hereditary sensory neuropathy-deafness-dementia syndrome / Orphanet_456318