IMPC phenotypes (gene matching)
C57BL/6-Il1atm1.1Itl/H
| Status | Available to order |
| EMMA ID | EM:15191 |
| Citation information | RRID:IMSR_EM:15191 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Il1atm1.1Itl/H |
| Alternative name | IL-1aTM |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Il1atm1.1Itl |
| Gene/Transgene symbol | Il1a |
Information from provider
| Provider | Murray Clarke |
| Provider affiliation | Cardiorespiratory Medicine, Cambridge University |
| Genetic information | IL-1alphaTM mice were generated by homologous recombination of the base pairs in exon 5 of Il1a gene to create the point mutation R114Q in a C57BL/6 FLP ES cell line, using an FRT flanked neo selection cassette, and generation of chimaeras with C57BL/6J mice. The resultant R114Q pro-IL1A protein mutant cannot be cleaved and activated by thrombin. |
| Phenotypic information | Homozygous:Mice bearing a mutation in the IL-1alpha thrombin cleavage site (R114Q) exhibited defects in efficient wound healing and rapid thrombopoiesis after acute platelet loss.Heterozygous:No phenotypic difference from wild-type mice. |
| Breeding history | IL-1alphaTM mice were born at expected frequencies with no gross phenotype compared to littermate controls. Mice are maintained as homozygotes (C57BL/6J). Currently on generation F17. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Literature references
- The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin.;Burzynski Laura C, Humphry Melanie, Pyrillou Katerina, Wiggins Kimberley A, Chan Julie N E, Figg Nichola, Kitt Lauren L, Summers Charlotte, Tatham Kate C, Martin Paul B, Bennett Martin R, Clarke Murray C H, ;2019;Immunity;50;1033-1042.e6; 30926232