C.FVB(B6)-Tg(Rorc-EGFP)1Ebe/Orl
| Status | Available to order |
| EMMA ID | EM:15250 |
| Citation information | RRID:IMSR_EM:15250 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C.FVB(B6)-Tg(Rorc-EGFP)1Ebe/Orl |
| Alternative name | BALB/C Rorc(gt)-GfpTG |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Rorc-EGFP)1Ebe |
| Gene/Transgene symbol | Tg(Rorc-EGFP)1Ebe |
Information from provider
| Provider | Kamel Benlagha |
| Provider affiliation | Immunology, IRSL-Inserm U1160 |
| Genetic information | The coding sequence for EGFP, including the stop codon, was inserted into exon 1 of Rorc (gamma-t, RORgt) in place of the endogenous ATG translation start codon, on a 200-kb BAC (Invitrogen) carrying at least 70 kb of sequence upstream of the Rorc (gamma-t) translation start site. Pronucleus of FVB/N fertilized eggs were used, and transgenic mice were backcrossed 10 times to C57BL/6 mice. |
| Phenotypic information | Homozygous:Cells expressing RORgt are GFP-positive.Heterozygous:Cells expressing RORgt are GFP-positive. |
| Breeding history | Mice were generated on FVB/N background, backcrossed 10 times to C57BL/6 and, from the C57BL/6 background, backcrossed in our laboratory more than 15 times to mice on a BALB/c genetic background. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous BALB/c males |
Literature references
- Characterization of the developmental landscape of murine RORγt+ iNKT cells.;Klibi Jihene, Li Shamin, Amable Ludivine, Joseph Claudine, Brunet Stéphane, Delord Marc, Parietti Veronique, Jaubert Jean, Marie Julien, Karray Saoussen, Eberl Gerard, Lucas Bruno, Toubert Antoine, Benlagha Kamel, ;2020;International immunology;32;105-116; 31565740