B6;129S6-Tg(CAG-Bmi1,-EGFP)IB5Mro/H
| Status | Available to order |
| EMMA ID | EM:15259 |
| Citation information | RRID:IMSR_EM:15259 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129S6-Tg(CAG-Bmi1,-EGFP)IB5Mro/H |
| Alternative name | STOPFloxBmi1/IB5 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-Bmi1,-EGFP)IB5Mro |
| Gene/Transgene symbol | Tg(CAG-Bmi1,-EGFP)IB5Mro |
Information from provider
| Provider | Silvia Marino |
| Provider affiliation | Centre for Genomic and Child Health, Blizard |
| Genetic information | Conditional gain-of-function expression of Bmi1. The pccall2 construct was chosen to drive conditional expression of Bmi1. It is composed of a cytomegalovirus (CMV) enhancer/chicken β-actin promoter followed by a βgeo (LacZ/neomycin) fusion gene and three copies of the SV40 polyA signal flanked by LoxP sites. The full-length murine Bmi1 cDNA was inserted after the second LoxP site, in front of an IRES-eGFP (enhanced green fluorescent protein) sequence. The CMV enhancer/chicken β-actin promoter drives β-galactosidase expression and confers neomycin resistance, however, on cre-mediated excision of βgeo, Bmi1 and eGFP will be simultaneously expressed. The pccall2-Bmi1 vector was introduced into TC-1 mouse ES cells. Four ES cell clones showing high LacZ expression and single copy integration were selected and electroporated with a cre recombinase expression vector to test the induction of Bmi1 overexpression. Clones IB5 and IE1 showed negative LacZ expression and Bmi1 protein level comparable with a medulloblastoma sample. They were therefore selected for injection into blastocysts to generate chimeric mice. Germline transmission and line establishment was achieved for clone IB5. This line is referred to as STOPFloxBmi1. |
| Phenotypic information | Homozygous:Mice were not bred to homozygosity.Heterozygous:No obvious phenotype was observed; overexpression of Bmi1 will only be obtained upon crossing with a cre recombinase-expressing line. |
| Breeding history | IB5 males have been crossed with C57BL/6 wild-type females over many generations to obtain heterozygous offsprings to maintain the line. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Conditional activation of Bmi1 expression regulates self-renewal, apoptosis, and differentiation of neural stem/progenitor cells in vitro and in vivo.;Yadirgi Gokhan, Leinster Veronica, Acquati Serena, Bhagat Heeta, Shakhova Olga, Marino Silvia, ;2011;Stem cells (Dayton, Ohio);29;700-12; 21305672