B6.C(C3H)-Pde4b^enu1H/H

Status	Available to order
EMMA ID	EM:15265
Citation information	RRID:IMSR_EM:15265 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.C(C3H)-Pde4b^enu1H/H
Alternative name	B6.C-Pde4b enu1H (a.k.a. Pde4b-Y358C; MGI: 5648042)
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Pde4b^enu1H
Gene/Transgene symbol	Pde4b

Information from provider

Provider	Steven Clapcote
Provider affiliation	School of Biomedical Sciences, University of Leeds
Additional owner	MRC Harwell.
Genetic information	ENU mutagenesis induced an adenine to guanine (A1073G) transition in Pde4b exon 11 that results in the amino acid substitution of cysteine for tyrosine at position 358 (Y358C).
Phenotypic information	Homozygous: In behavioral assays, B6.C-Pde4b mice display decreased anxiety and increased exploration, as well as cognitive enhancement across several tests of learning and memory, consistent with synaptic changes including enhanced long-term potentiation and impaired depotentiation ex vivo. B6.C-Pde4b mice also demonstrate enhanced neurogenesis. Heterozygous: Superficially normal, but a detailed analysis of the heterozygous phenotype has not been carried out.
Breeding history	The mutation was induced by ENU in a BALB/cAnN mouse and was first detected within that mouse's (C3H/HeH × BALB/cAnN) F1 progeny (mouse stock EMRCB/60.3d). It was subsequently outcrossed once to C57BL/6NTac and then maintained by backcrossing to C57BL/6JCrl (Charles River) for at least 10 generations.
References	Specific Inhibition of Phosphodiesterase-4B Results in Anxiolysis and Facilitates Memory Acquisition.;McGirr Alexander, Lipina Tatiana V, Mun Ho-Suk, Georgiou John, Al-Amri Ahmed H, Ng Enoch, Zhai Dongxu, Elliott Christina, Cameron Ryan T, Mullins Jonathan G L, Liu Fang, Baillie George S, Clapcote Steven J, Roder John C, ;2016;Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology;41;1080-92; 26272049
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal spinal cord morphology / MGI
decreased anxiety-related response / MGI
increased exploration in new environment / MGI
abnormal object recognition memory / MGI
abnormal learning/memory/conditioning / MGI
abnormal CNS synaptic transmission / MGI
increased vertical activity / MGI
decreased synaptic depression / MGI
increased post-tetanic potentiation / MGI
enhanced long term potentiation / MGI
nervous system phenotype / MGI
cardiovascular system phenotype / MGI
behavior/neurological phenotype / MGI
taste/olfaction phenotype / MGI
brain vacuoles / MGI
abnormal spatial working memory / MGI
decreased tumor necrosis factor secretion / MGI
impaired neutrophil recruitment / MGI
impaired neutrophil chemotaxis / MGI
impaired cued conditioning behavior / MGI
decreased sensitivity to induced morbidity/mortality / MGI
abnormal neuron differentiation / MGI
enhanced learning / MGI

Literature references

Specific Inhibition of Phosphodiesterase-4B Results in Anxiolysis and Facilitates Memory Acquisition.;McGirr Alexander, Lipina Tatiana V, Mun Ho-Suk, Georgiou John, Al-Amri Ahmed H, Ng Enoch, Zhai Dongxu, Elliott Christina, Cameron Ryan T, Mullins Jonathan G L, Liu Fang, Baillie George S, Clapcote Steven J, Roder John C, ;2016;Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology;41;1080-92; 26272049

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B6.C(C3H)-Pde4benu1H/H