B6J.Cg-Armcx3tm1.1Eso/Cnbc
| Status | Available to order |
| EMMA ID | EM:15434 |
| Citation information | RRID:IMSR_EM:15434 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.Cg-Armcx3tm1.1Eso/Cnbc |
| Alternative name | Armcx3fl/fl ES |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Armcx3tm1.1Eso |
| Gene/Transgene symbol | Armcx3 |
Information from provider
| Provider | Eduardo Soriano |
| Provider affiliation | University of Barcelona |
| Genetic information | The targeted gene is the armadillo repeat containing, X-linked 3 (Armcx3). The Armcx3 knock-out targeting construct was designed and constructed at the IRB Mouse Mutant Core Facility using standard gene targeting recombineering methods. The targeting vector was confirmed by sequencing and the linearized vector transfected into ES cells for targeting. Cells were selected by neomycin resistance and genomic DNA analyzed by PCR and by Southern blot for correct targeting. PCR analysis was used for screening the 5′ and 3′ insertion sites of the Armcx3 knock-out allele. Digested genomic DNA was analyzed following SacI digestion by southern analysis and correctly targeted clones were injected into C57BL/6J blastocysts and reimplanted into the oviduct of 2.5-day CD1 pseudopregnant foster mice. Chimeras were mated with heterozygous flp deleter mice to remove the neomycin selection marker and to obtain the floxed-Armcx3 mice. |
| Phenotypic information | Homozygous:Homozygous mice will have the exon 5 of the Armcx3 gene flanked by loxP sequences. No evident phenotypic alterations have been observed in these conditions. A clear phenotype is likely to appear in experimental settings where the Armcx3 gene is inactivated during embryonic stages. Less dramatic phenotypes have been observed when Armcx3 is inactivated at early postnatal stages or adult stages.Heterozygous:Given that the gene is located in the X-chromosome, heterozygous mice are always females. No evident phenotypic alterations have been observed. |
| Breeding history | The mutant mice have been backcrossed more than 10 times to C57BL/6J mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity.;Mirra Serena, Gavaldà-Navarro Aleix, Manso Yasmina, Higuera Mónica, Serrat Román, Salcedo María Teresa, Burgaya Ferran, Balibrea José Maria, Santamaría Eva, Uriarte Iker, Berasain Carmen, Avila Matias A, Mínguez Beatriz, Soriano Eduardo, Villarroya Francesc, ;2021;Cancers;13;; 33807672