B6.129(Cg)-Ube2i^tm2.1Adej/Orl

Status	Available to order
EMMA ID	EM:15574
Citation information	RRID:IMSR_EM:15574 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129(Cg)-Ube2i^tm2.1Adej/Orl
Alternative name	Ubc9flx/flx
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Ube2i^tm2.1Adej
Gene/Transgene symbol	Ube2i

Information from provider

Provider	Anne DEJEAN
Provider affiliation	Institut Pasteur
Genetic information	The Ube2i (Ubc9) floxed allele was generated by flanking exon 2, which contains the initiator ATG, and exon 3 with loxP sites as published in "Sumoylation by Ubc9 Regulates the Stem Cell Compartment and Structure and Function of the Intestinal Epithelium in Mice" Demarque Maud et al., Gastroenterology, 2011 (doi:10.1053/j.gastro.2010.10.002).
Phenotypic information	Homozygous: Absence of visible phenotype. Heterozygous: Absence of visible phenotype.
Breeding history	10 backcrosses to C57BL/6J.
References	Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes.;Wang Aibo, Ding Xiao, Demarque Maud, Liu Xindong, Pan Deng, Xin Huawei, Zhong Bo, Wang Xiaohu, Dejean Anne, Jin Wei, Dong Chen, ;2017;Journal of immunology (Baltimore, Md. : 1950);198;3461-3470; 28314856 SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.;Cossec Jack-Christophe, Theurillat Ilan, Chica Claudia, Búa Aguín Sabela, Gaume Xavier, Andrieux Alexandra, Iturbide Ane, Jouvion Gregory, Li Han, Bossis Guillaume, Seeler Jacob-Sebastian, Torres-Padilla Maria-Elena, Dejean Anne, ;2018;Cell stem cell;23;742-757.e8; 30401455 Sumoylation coordinates the repression of inflammatory and anti-viral gene-expression programs during innate sensing.;Decque Adrien, Joffre Olivier, Magalhaes Joao G, Cossec Jack-Christophe, Blecher-Gonen Ronnie, Lapaquette Pierre, Silvin Aymeric, Manel Nicolas, Joubert Pierre-Emmanuel, Seeler Jacob-Sebastian, Albert Matthew L, Amit Ido, Amigorena Sebastian, Dejean Anne, ;2016;Nature immunology;17;140-9; 26657003 Protein SUMOylation Is Required for Regulatory T Cell Expansion and Function.;Ding Xiao, Wang Aibo, Ma Xiaopeng, Demarque Maud, Jin Wei, Xin Huawei, Dejean Anne, Dong Chen, ;2016;Cell reports;16;1055-1066; 27425617 Sumoylation by Ubc9 regulates the stem cell compartment and structure and function of the intestinal epithelium in mice.;Demarque Maud D, Nacerddine Karim, Neyret-Kahn Hélène, Andrieux Alexandra, Danenberg Esther, Jouvion Grégory, Bomme Perrine, Hamard Ghislaine, Romagnolo Béatrice, Terris Benoît, Cumano Ana, Barker Nick, Clevers Hans, Dejean Anne, ;2011;Gastroenterology;140;286-96; 20951138 Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in mice.;Rodriguez Amanda, Briley Shawn M, Patton Bethany K, Tripurani Swamy K, Rajapakshe Kimal, Coarfa Cristian, Rajkovic Aleksander, Andrieux Alexandra, Dejean Anne, Pangas Stephanie A, ;2019;Development (Cambridge, England);146;328-341.e5; 31704792 Febrile Temperature Critically Controls the Differentiation and Pathogenicity of T Helper 17 Cells.;Wang Xiaohu, Ni Lu, Wan Siyuan, Zhao Xiaohong, Ding Xiao, Dejean Anne, Dong Chen, ;2020;Immunity;52;1141-1159.e7; 32049050 SUMOylation of Enzymes and Ion Channels in Sensory Neurons Protects against Metabolic Dysfunction, Neuropathy, and Sensory Loss in Diabetes.;Agarwal Nitin, Taberner Francisco J, Rangel Rojas Daniel, Moroni Mirko, Omberbasic Damir, Njoo Christian, Andrieux Alexandra, Gupta Pooja, Bali Kiran K, Herpel Esther, Faghihi Faramarz, Fleming Thomas, Dejean Anne, Lechner Stefan G, Nawroth Peter P, Lewin Gary R, Kuner Rohini, ;2020;Neuron;107;; 32735781
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archiving	homozygous C57BL/6J males

Disease and phenotype information

IMPC phenotypes (gene matching)

increased circulating calcium level / IMPC
decreased erythrocyte cell number / IMPC
increased leukocyte cell number / IMPC
embryonic lethality prior to organogenesis / IMPC
increased mean corpuscular hemoglobin / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal locomotor behavior / IMPC
abnormal vocalization / IMPC
increased mean corpuscular volume / IMPC
increased circulating phosphate level / IMPC

Literature references

Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes.;Wang Aibo, Ding Xiao, Demarque Maud, Liu Xindong, Pan Deng, Xin Huawei, Zhong Bo, Wang Xiaohu, Dejean Anne, Jin Wei, Dong Chen, ;2017;Journal of immunology (Baltimore, Md. : 1950);198;3461-3470; 28314856
SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.;Cossec Jack-Christophe, Theurillat Ilan, Chica Claudia, Búa Aguín Sabela, Gaume Xavier, Andrieux Alexandra, Iturbide Ane, Jouvion Gregory, Li Han, Bossis Guillaume, Seeler Jacob-Sebastian, Torres-Padilla Maria-Elena, Dejean Anne, ;2018;Cell stem cell;23;742-757.e8; 30401455
Sumoylation coordinates the repression of inflammatory and anti-viral gene-expression programs during innate sensing.;Decque Adrien, Joffre Olivier, Magalhaes Joao G, Cossec Jack-Christophe, Blecher-Gonen Ronnie, Lapaquette Pierre, Silvin Aymeric, Manel Nicolas, Joubert Pierre-Emmanuel, Seeler Jacob-Sebastian, Albert Matthew L, Amit Ido, Amigorena Sebastian, Dejean Anne, ;2016;Nature immunology;17;140-9; 26657003
Protein SUMOylation Is Required for Regulatory T Cell Expansion and Function.;Ding Xiao, Wang Aibo, Ma Xiaopeng, Demarque Maud, Jin Wei, Xin Huawei, Dejean Anne, Dong Chen, ;2016;Cell reports;16;1055-1066; 27425617
Sumoylation by Ubc9 regulates the stem cell compartment and structure and function of the intestinal epithelium in mice.;Demarque Maud D, Nacerddine Karim, Neyret-Kahn Hélène, Andrieux Alexandra, Danenberg Esther, Jouvion Grégory, Bomme Perrine, Hamard Ghislaine, Romagnolo Béatrice, Terris Benoît, Cumano Ana, Barker Nick, Clevers Hans, Dejean Anne, ;2011;Gastroenterology;140;286-96; 20951138
Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in mice.;Rodriguez Amanda, Briley Shawn M, Patton Bethany K, Tripurani Swamy K, Rajapakshe Kimal, Coarfa Cristian, Rajkovic Aleksander, Andrieux Alexandra, Dejean Anne, Pangas Stephanie A, ;2019;Development (Cambridge, England);146;328-341.e5; 31704792
Febrile Temperature Critically Controls the Differentiation and Pathogenicity of T Helper 17 Cells.;Wang Xiaohu, Ni Lu, Wan Siyuan, Zhao Xiaohong, Ding Xiao, Dejean Anne, Dong Chen, ;2020;Immunity;52;1141-1159.e7; 32049050
SUMOylation of Enzymes and Ion Channels in Sensory Neurons Protects against Metabolic Dysfunction, Neuropathy, and Sensory Loss in Diabetes.;Agarwal Nitin, Taberner Francisco J, Rangel Rojas Daniel, Moroni Mirko, Omberbasic Damir, Njoo Christian, Andrieux Alexandra, Gupta Pooja, Bali Kiran K, Herpel Esther, Faghihi Faramarz, Fleming Thomas, Dejean Anne, Lechner Stefan G, Nawroth Peter P, Lewin Gary R, Kuner Rohini, ;2020;Neuron;107;; 32735781

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B6.129(Cg)-Ube2itm2.1Adej/Orl