| Status | Available to order |
| EMMA ID | EM:15575 |
| Citation information | RRID:IMSR_EM:15575 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.B6N(129S7)-Ldlrtm1Her Apobtm2Sgy Tg(Csf1r-EGFP/Rpl10a)1Glass/Oulu |
| Alternative name | cfms-EGFP-L10a/Ldlr- ApoB100only |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Apobtm2Sgy, Tg(Csf1r-EGFP/Rpl10a)1Glass, Ldlrtm1Her |
| Gene/Transgene symbol | Apob, Tg(Csf1r-EGFP/Rpl10a)1Glass, Ldlr |
| Provider | Minna Kaikkonen-Määttä |
| Provider affiliation | A.I. Virtanen -instituutti, University of Eastern Finland |
| Additional owner | The original cfms-L10a-EGFP strain was developed by Prof. Christopher Glass at University of California San Diego. The L10a-EGFP construct was provided by Prof. Nathaniel Heintz, Howard Hughes Medical Institute/The Rockefeller University. |
| Genetic information | The construct expressing L10alpha ribosomal subunit (Rpl10a) fused to EGFP was obtained from N. Heintz (Rockfeller U. NY). The EGFP-L10alpha construct was then cloned downstream of the Csf1r (c-fms) promoter (Sasmono et al., Blood 2003, doi:10.1182/blood-2002-02-0569) and the resulting vector injected into the pronuclei of fertilized oocytes. The EGFP-L10alpha fusion protein is constitutively expressed in myeloid cells. The EGFP-L10alpha transgenic mice were crossed to the double-mutant strain for Ldlr and ApoB (The Jackson Laboratory, strain #003000). Expression of EGFP-L10alpha allows to specifically immunoprecipitate polysome bound mRNA in myeloid cells in atherosclerosis diseases. |
| Phenotypic information | Homozygous:The expression of EGFPL10alpha allows to specifically immunprecipitate polysome bound mRNA in myeloid cells in atherosclerosis disease.Heterozygous:The expression of EGFPL10alpha allows to specifically immunprecipitate polysome bound mRNA in myeloid cells in atherosclerosis disease. |
| Breeding history | Transgenic mice were generated in the C57BL/6NHsd (code 044) genetic background and have been bred to C57BL/6JOlaHsd since 2013. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | University of Oulu, Oulu, Finland |
| Animals used for archiving | homozygous males |
| Stage of embryos | 2-cell |
Orphanet associated rare diseases, based on orthologous gene matching
