C57BL/6;129-Tet2tm1.2RaoCxcl10tm1AdlJak2tm1(JAK2)Argr/H
| Status | Available to order |
| EMMA ID | EM:15668 |
| Citation information | RRID:IMSR_EM:15668 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6;129-Tet2tm1.2RaoCxcl10tm1AdlJak2tm1(JAK2)Argr/H |
| Alternative name | B6.129S4-Cxcl10tm1Adl/J-Jak2tmV617F-Tet2tm1.2Rao/J |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Cxcl10tm1Adl, Tet2tm1.2Rao, Jak2tm1(JAK2)Argr |
| Gene/Transgene symbol | Cxcl10, Tet2, Jak2 |
Information from provider
| Provider | David Kent |
| Provider affiliation | University of York |
| Additional owner | see above |
| Genetic information | This strain is a disease model for Myeloproliferative neoplasm (MPN) and is heterozygous for a point mutation (V617F) in the gene Jak2 and homozygous knockout for Tet2 and Cxcl10. This strain was generated by crossing a Cxcl10 knockout with an allelic series of Jak2 (V617F) knock-in and a Tet2 knockout to study the impact of Cxcl10 on red cell phenotypes associated with MPN pathogenesis. |
| Phenotypic information | Homozygous:Triple mutant mice have a partial amelioration of disease phenotype compared to JAK2 single mutant and JAK2 TET2 double mutant mice. JAK2 single mutant mice display erythrocytosis.TET2 single mutant knockout mice display increased monocytes and increased HSC self-renewal. Cxcl10 single mutant knockout mice have a T cell defect. JAK2 TET2 double mutant mice display a more severe myeloproliferative phenotype with durable HSC self-renewal.Heterozygous:JAK2 heterozygous mice have a reduced phenotype that is shifted towards platelet, rather than erythroid cell, production. |
| Breeding history | B6.JAK2tmV617F heterozygous mice were crossed to B6.129S4-Cxcl10tm1 homozygous mice. B6.JAK2+/tmV617F-Cxcl10tm1-/- mice were then crossed to B6.Tet2tm1.2Rao/J homozygous mice and F1 mice were crossed to create a final strain of B6.129S4-Cxcl10tm1Adl/J-Jak2tmV617F-Tet2tm1.2Rao/J mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Increased CXCL10 (IP-10) is associated with advanced myeloproliferative neoplasms and its loss dampens erythrocytosis in mouse models.;Belmonte Miriam, Cabrera-Cosme Lilia, Øbro Nina F, Li Juan, Grinfeld Jacob, Milek Joanna, Bennett Ellie, Irvine Melissa, Shepherd Mairi S, Cull Alyssa H, Boyd Grace, Riedel Lisa M, Chi Che James Lok, Oedekoven Caroline A, Baxter E Joanna, Green Anthony R, Barlow Jillian L, Kent David G, ;2024;Experimental hematology;135;104246; 38763471