IMPC phenotypes (gene matching)
129-Dlgap4Gt(CA0132)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15817 |
| Citation information | RRID:IMSR_EM:15817 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Dlgap4Gt(CA0132)Wtsi/WtsiH |
| Alternative name | 129-Dlgap4 |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Dlgap4Gt(CA0132)Wtsi |
| Gene/Transgene symbol | Dlgap4 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for dhttps://doi.org/10.1101/500389:This mouse strain was created via ES cells to mouse conversion. The ES cells (CA0132, strain 129/Ola) were obtained from Sanger Institute Gene Trap Resource (SIGTR) An insertional mutation in Dlgap4(CA0132) was inserted in mouse ES cells using gene trap vector pGT0lxr. The gene trap, located in introns 8-9, creates an in-frame fusion between the 5' exons of the trapped gene and a reporter, Beta-geo in exon 1-8. |
| Phenotypic information | Mutant mice showed medium overall behavioural difference from wildtypes, with seven of 16 behaviour variables significantly impacted in these mutants. In the elevated plus maze task, three behavioural variables of five were significantly impacted in mutants. In the open field/novel object exploration task, one behavioural variable, OF NOE total distance, was significantly increased in mutants. In the fear learning task, one behavioural variable, fear learning tone effect, was significantly decreased in mutants. In the contextual memory task, one behavioural variable, contextual memory change, was significantly decreased in mutants. In the cued memory task, one behavioural variable, cued memory change, was significantly decreased in mutants. Only the rotarod task was unaffected |
| Breeding history | Mice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |