129S5;129P2-Akap9^{Gt(XP0050)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:15818
Citation information	RRID:IMSR_EM:15818 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	129S5;129P2-Akap9^{Gt(XP0050)Wtsi}/WtsiH
Alternative name	129-Akap9/H
Strain type	Gene-trap
Allele/Transgene symbol	Akap9^{Gt(XP0050)Wtsi}
Gene/Transgene symbol	Akap9

Information from provider

Provider	Seth Grant
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	Information sourced from supplementary data 1 for dhttps://doi.org/10.1101/500389: This mouse strain was created via ES cells to mouse conversion. The ES (XP0050, strain 129/Ola) cells were obtained from Sanger Institute Gene Trap Resource (SIGTR) An insertional mutation in Gnb1(AN0901) was inserted in mouse ES cells using gene trap vector pGT0lxr. The gene trap, located in intron 14-15, creates an in-frame fusion between the 5' exons of the trapped gene and a reporter, Beta-geo in exons 1-14.
Phenotypic information	Mutant mice showed no discernible overall behavioural difference from wildtypes, with two of 16 behaviour variables significantly impacted by this mutation. In the fear learning task, one behavioural variable, fear learning tone effect, was significantly decreased in mutants. In the contextual memory task, one behavioural variable, contextual memory change, was significantly decreased in mutants. Elevated plus maze, open field/novel object exploration, rotarod and cued memory tasks were unaffected
Breeding history	Mice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study.
References	None available
Homozygous fertile	not known
Homozygous viable	yes
Homozygous matings required	not known
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Romano-Ward syndrome / Orphanet_101016

IMPC phenotypes (gene matching)

decreased circulating cholesterol level / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased body weight / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating serum albumin level / IMPC
decreased total body fat amount / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased circulating amylase level / IMPC
increased bone mineral density / IMPC
abnormal head morphology / IMPC

MGI phenotypes (gene matching)

decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
decreased circulating calcium level / MGI
abnormal head morphology / MGI
abnormal testis morphology / MGI
arrest of spermatogenesis / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
male infertility / MGI
abnormal tooth morphology / MGI
abnormal seminiferous tubule morphology / MGI
globozoospermia / MGI
oligozoospermia / MGI
increased circulating alkaline phosphatase level / MGI
decreased lean body mass / MGI
abnormal Sertoli cell development / MGI
abnormal pelvic girdle bone morphology / MGI
decreased lumbar vertebrae number / MGI
decreased testis weight / MGI
increased energy expenditure / MGI
decreased seminal vesicle weight / MGI
increased T cell number / MGI
decreased B cell number / MGI
azoospermia / MGI
decreased circulating cholesterol level / MGI
increased oxygen consumption / MGI
decreased circulating serum albumin level / MGI
decreased percent body fat/body weight / MGI
decreased circulating glucose level / MGI
decreased circulating total protein level / MGI
abnormal spermatocyte morphology / MGI
increased CD4-positive, alpha beta T cell number / MGI
increased CD8-positive, alpha-beta T cell number / MGI
decreased mature B cell number / MGI
arrest of male meiosis / MGI
increased carbon dioxide production / MGI
decreased total body fat amount / MGI
increased circulating fructosamine level / MGI
decreased circulating fructosamine level / MGI
lethality, incomplete penetrance / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

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129S5;129P2-Akap9Gt(XP0050)Wtsi/WtsiH