IMPC phenotypes (gene matching)
129-Sipa1l1tm1(IRES-lacZ-neo)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15822 |
| Citation information | RRID:IMSR_EM:15822 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Sipa1l1tm1(IRES-lacZ-neo)Wtsi/WtsiH |
| Alternative name | 129-Sipa1l1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sipa1l1tm1(IRES-lacZ-neo)Wtsi |
| Gene/Transgene symbol | Sipa1l1 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for dhttps://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 1.2kb of Sipa1l1 genomic DNA (X83442041 to X83443195; Ensemble Build 50) with IRES-lacZ-neo cassette in exon 5 leading to a frameshift mutation between exon 5 and 6. |
| Phenotypic information | Mutant mice showed little overall behavioural difference from wildtypes, with two of 16 behaviour variables significantly impacted in these mutants. In the rotarod task, one behavioural variable, RR memory, was significantly increased in mutants. In the cued memory task, one behavioural variable, cued memory mean, was significantly increased in mutants. Elevated plus maze, open field/novel object exploration, fear learning and contextual memory tasks were unaffected. |
| Breeding history | Mice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |