129-Igsf9tm1(IRES-lacZ-neo)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:15824
Citation informationRRID:IMSR_EM:15824 

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International strain name129-Igsf9tm1(IRES-lacZ-neo)Wtsi/WtsiH
Alternative name129-Igsf9/WtsiH
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolIgsf9tm1(IRES-lacZ-neo)Wtsi
Gene/Transgene symbolIgsf9

Information from provider

ProviderSeth Grant
Provider affiliationWellcome Trust Sanger Institute
Genetic informationInformation sourced from supplementary data 1 for dhttps://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 1.7kb of Igsf9 genomic DNA (X174419685 to X174421368; Ensemble Build 55) with IRES-lacZ-neo cassette in exon 4-6 leading to a frameshift mutation between exon 3 and 7.
Phenotypic informationMutant mice showed little overall behavioural difference from wildtypes, with no behavioural variables significantly affected by this mutation.
Breeding historyMice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study.
ReferencesNone available
Homozygous fertilenot known
Homozygous viableyes
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased circulating creatinine level / IMPC
  • increased grip strength / IMPC
  • increased circulating phosphate level / IMPC

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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Practical information

Genotyping protocol

Example health report
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