129-Iqsec3tm1(IRES-lacZ-neo)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:15825
Citation informationRRID:IMSR_EM:15825 

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International strain name129-Iqsec3tm1(IRES-lacZ-neo)Wtsi/WtsiH
Alternative name129-Iqsec3/WtsiH
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolIqsec3tm1(IRES-lacZ-neo)Wtsi
Gene/Transgene symbolIqsec3

Information from provider

ProviderSeth Grant
Provider affiliationWellcome Trust Sanger Institute
Genetic informationInformation sourced from supplementary data 1 for dhttps://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced This replaced 3kb of Iqsec3 genomic DNA (X121360567 to X121363526; Ensemble Build 55) with IRES-lacZ-neo cassette in exon 4-5 leading to a frameshift mutation between exon 3 and 6.
Phenotypic informationMutant mice showed medium overall behavioural difference from wildtypes, with eight of 16 behaviour variables significantly impacted in these mutant mice. In the elevated plus maze task, four behavioural variables of five were significantly impacted in mutants. In the open field/novel object exploration task, one behavioural variable, OF NOE total distance, was significantly increased in mutants. In the fear learning task, two behavioural variables of two were significantly impacted in mutants. In the contextual memory task, one behavioural variable, contextual memory mean, was significantly decreased in mutants. Only rotarod and cued memory tasks were unaffected.
Breeding historyMice were maintained by backcrossing onto the 129S5/ SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study.
ReferencesNone available
Homozygous fertilenot known
Homozygous viableyes
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased body length / IMPC
  • decreased bone mineral content / IMPC
  • decreased prepulse inhibition / IMPC
  • decreased bone mineral density / IMPC
  • decreased lean body mass / IMPC
  • decreased locomotor activity / IMPC
  • decreased thigmotaxis / IMPC
  • increased total body fat amount / IMPC
  • increased fasting circulating glucose level / IMPC
  • decreased total retina thickness / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal retina inner nuclear layer morphology / IMPC

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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Practical information

Genotyping protocol

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