IMPC phenotypes (gene matching)
129-Tspyl2tm1(IRES-lacZ-neo)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15831 |
| Citation information | RRID:IMSR_EM:15831 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Tspyl2tm1(IRES-lacZ-neo)Wtsi/WtsiH |
| Alternative name | 129-Tspyl2 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Tspyl2tm1(IRES-lacZ-neo)Wtsi |
| Gene/Transgene symbol | Tspyl2 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for https://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 3.5kb of Tspyl2 genomic DNA (X148773141 to X148776721; Ensemble Build 56) with IRES-lacZ-neo cassette in exons 2-6 leading to a frameshift mutation between exon 1 and 7. |
| Phenotypic information | Mutant mice showed large overall behavioural difference from wildtypes, with five of 16 behaviour variables. significantly impacted in these mutant mice. In the elevated plus maze task, two behavioural variables of five were significantly impacted in mutants. In the open field/novel object exploration task, one behavioural variable, OF NOE total distance, was significantly increased in mutants. In the rotarod task, one behavioural variable, RR naive fall time, was significantly decreased in mutants. In the cued memory task, one behavioural variable, cued memory change, was significantly increased in mutants. Only fear learning and contextual memory tasks were unaffected. |
| Breeding history | Backcrosses onto the 129S5/SvEvBrd background were used to maintain the colony and to generate hemizygous and wildtype mice to study. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |