129-Sorbs2tm1(IRES-lacZ-neo)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:15833
Citation informationRRID:IMSR_EM:15833 

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International strain name129-Sorbs2tm1(IRES-lacZ-neo)Wtsi/WtsiH
Alternative name129-Sorbs2/WtsiH
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolSorbs2tm1(IRES-lacZ-neo)Wtsi
Gene/Transgene symbolSorbs2

Information from provider

ProviderSeth Grant
Provider affiliationWellcome Trust Sanger Institute
Genetic informationInformation sourced from supplementary data 1 for https://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 940bp of Sorbs2 genomic DNA (X46855222 to X46856162; Ensemble Build 55) with IRES-lacZ-neo cassette in exons 9-10 leading to a frameshift mutation between exon 8 and 11.
Phenotypic informationMutant mice showed little overall behavioural difference from wildtypes, with four of 16 behaviour variables significantly impacted in these mutant mice. In the elevated plus maze task, one behavioural variable, EPM total distance, was significantly decreased in mutants. In the open field/novel object exploration task, one behavioural variable, OF NOE total distance, was significantly decreased in mutants. In the rotarod task, one behavioural variable, RR naive fall time, was significantly decreased in mutants. In the fear learning task, one behavioural variable, fear learning tone effect, was significantly decreased in mutants. Only contextual memory and cued memory tasks were unaffected.
Breeding historyMice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study.
ReferencesNone available
Homozygous fertilenot known
Homozygous viableyes
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased circulating free fatty acids level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • abnormal retina morphology / IMPC
  • shortened PQ interval / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • increased heart weight / IMPC
  • decreased circulating glucose level / IMPC
  • increased heart rate / IMPC
  • decreased circulating cholesterol level / IMPC
  • shortened ST segment / IMPC
  • increased circulating sodium level / IMPC
  • decreased exploration in new environment / IMPC
  • shortened PR interval / IMPC

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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Practical information

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