129-Git1tm1(IRES-lacZ-neo)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15835 |
| Citation information | RRID:IMSR_EM:15835 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Git1tm1(IRES-lacZ-neo)Wtsi/WtsiH |
| Alternative name | 129-Git1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Git1tm1(IRES-lacZ-neo)Wtsi |
| Gene/Transgene symbol | Git1 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for https://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 1.1 kb of Git1 genomic DNA (X77312499 to X77313637; Ensemble Build 55) with IRES-lacZ-neo cassette in exons 5-6 leading to a frameshift mutation between exon 4 and 7. |
| Phenotypic information | Mutant mice showed little overall behavioural difference from wildtypes, with three of 16 behaviour vari#2;ables significantly impacted in these mutant mice. In the elevated plus maze task, two behavioural variables of five were significantly impacted in mutants. In the rotarod task, one behavioural variable, RR naive fall time, was significantly decreased in mutants. Open field/novel object exploration, fear learning, contextual memory and cued memory tasks were unaffected. |
| Breeding history | Mice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous, heterozygous and wildtype mice to study |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |