IMPC phenotypes (gene matching)
129-Dlgap2tm1(IRES-lacZ-neo)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15840 |
| Citation information | RRID:IMSR_EM:15840 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Dlgap2tm1(IRES-lacZ-neo)Wtsi/WtsiH |
| Alternative name | 129-Dlgap2 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Dlgap2tm1(IRES-lacZ-neo)Wtsi |
| Gene/Transgene symbol | Dlgap2 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for https://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 1.8kb of Dlgap2 genomic DNA (X14822401 to X14824243; Ensemble Build 69) with IRES-lacZ-neo cassette in exon 9-10 leading to a frameshift mutation between exon 8 and 11. |
| Phenotypic information | Mice showed little overall behavioural difference from wildtypes, with two of 16 behaviour variables significantly impacted in these mutant mice. In the open field/novel object exploration task, one behavioural variable, OF NOE total distance, was significantly decreased in mutants. In the rotarod task, one behavioural variable, RR naive fall time, was significantly increased in mutants. Elevated plus maze, fear learning, con#2;textual memory and cued memory tasks were unaffected. |
| Breeding history | Mice were maintained by backcrossing onto the 129S5/ SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |