IMPC phenotypes (gene matching)
129-Shank2tm1(IRES-lacZ-Neo)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15841 |
| Citation information | RRID:IMSR_EM:15841 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Shank2tm1(IRES-lacZ-Neo)Wtsi/WtsiH |
| Alternative name | 129-Shank2 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Shank2tm1(IRES-lacZ-Neo)Wtsi |
| Gene/Transgene symbol | Shank2 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for https://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which replaced 1.1kb of Shank2 genomic DNA (X144179906 to X144180964; Ensemble Build 75) with IRES-lacZ-neo cassette in exon 11 leading to a frameshift mutation between exon 11 and 12. |
| Phenotypic information | Mice showed medium overall behavioural difference from wildtypes, with three of 16 behaviour variables significantly impacted in these mutant mice. In the elevated plus maze task, two behavioural variables of five were significantly impacted in mutants. In the rotarod task, one behavioural variable, RR memory, was significantly decreased in mutants. Open field/novel object exploration, fear learning, contextual memory and cued memory tasks were unaffected. |
| Breeding history | Mice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |