IMPC phenotypes (gene matching)
129-Dlgap1tm1(IRES-lacZ-neo)Wtsi/WtsiH
| Status | Available to order |
| EMMA ID | EM:15842 |
| Citation information | RRID:IMSR_EM:15842 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Dlgap1tm1(IRES-lacZ-neo)Wtsi/WtsiH |
| Alternative name | 129-Dlgap1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Dlgap1tm1(IRES-lacZ-neo)Wtsi |
| Gene/Transgene symbol | Dlgap1 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from supplementary data 1 for https://doi.org/10.1101/500389: This knockout mouse strain was created via ES cells to mouse conversion. E14TG2a ES cells were targeted with a vector which 795bp of Dlgap1 genomic DNA (X70662625 to X70663420; Ensemble Build 68) with IRES-lacZ-neo cassette in exons 4 leading to a frameshift mutation between exon 4 and 5. |
| Phenotypic information | Mutant mice showed little overall behavioural difference from wildtypes, with three of 16 behaviour vari#2;ables significantly impacted by this mutation. In the rotarod task, one behavioural variable, RR learning, was significantly decreased in mutants. In the contextual memory task, two behavioural variables of two were significantly impacted in mutants. Elevated plus maze, open field/novel object exploration, fear learning and cued memory tasks were unaffected. |
| Breeding history | Mice were maintained by backcrossing onto the 129S5/SvEvBrd background; heterozygous males and females were fertile and used to set up intercrosses to generate homozygous and wildtype mice to study. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |