B6J;129-Dlg4tm3.1Grnt/WtsiH

Status

Available to order

EMMA IDEM:15857
Citation informationRRID:IMSR_EM:15857 

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International strain nameB6J;129-Dlg4tm3.1Grnt/WtsiH
Alternative nameB6J;129-Dlg4/H
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolDlg4tm3.1Grnt
Gene/Transgene symbolDlg4

Information from provider

ProviderSeth Grant
Provider affiliationWellcome Trust Sanger Institute
Genetic informationInformation sourced from PMID: 19455133: The strain carries a Tandem Affinity Purification (TAP) tag inserted upstream of the stop codon in exon 19 along with a floxed neo cassette. The neo cassette was then removed via cre mediated recombination.
Breeding historyMaintained as Hom x Hom before backcrossing Homs to C57BL/6J and maintinaing as Het x Het/WT. Line is Hom viable at 20% and Hom fertile.
References
  • Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins.;Fernández Esperanza, Collins Mark O, Uren Rachel T, Kopanitsa Maksym V, Komiyama Noboru H, Croning Mike D R, Zografos Lysimachos, Armstrong J Douglas, Choudhary Jyoti S, Grant Seth G N, ;2009;Molecular systems biology;5;269; 19455133
Homozygous fertilenot known
Homozygous viableyes
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • decreased locomotor activity / IMPC
  • decreased grip strength / IMPC
  • decreased body weight / IMPC
  • tremors / IMPC
  • increased lean body mass / IMPC
  • increased circulating sodium level / IMPC
  • decreased exploration in new environment / IMPC
  • increased bone mineral content / IMPC
  • decreased total body fat amount / IMPC
  • abnormal behavior / IMPC
  • decreased bone mineral content / IMPC
MGI phenotypes (gene matching)
  • abnormal sebaceous gland morphology / MGI
  • abnormal forebrain morphology / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • abnormal social investigation / MGI
  • abnormal anxiety-related response / MGI
  • increased anxiety-related response / MGI
  • decreased anxiety-related response / MGI
  • abnormal maternal nurturing / MGI
  • hypoactivity / MGI
  • impaired coordination / MGI
  • increased grooming behavior / MGI
  • abnormal spatial learning / MGI
  • abnormal contextual conditioning behavior / MGI
  • decreased startle reflex / MGI
  • impaired balance / MGI
  • increased circulating corticosterone level / MGI
  • abnormal pain threshold / MGI
  • abnormal learning/memory/conditioning / MGI
  • abnormal innervation / MGI
  • abnormal CNS synaptic transmission / MGI
  • abnormal nociception after inflammation / MGI
  • decreased vertical activity / MGI
  • abnormal discrimination learning / MGI
  • abnormal AMPA-mediated synaptic currents / MGI
  • abnormal glutamate-mediated receptor currents / MGI
  • abnormal NMDA-mediated synaptic currents / MGI
  • enhanced paired-pulse facilitation / MGI
  • enhanced long term potentiation / MGI
  • allodynia / MGI
  • nervous system phenotype / MGI
  • abnormal synaptic transmission / MGI
  • maternal effect / MGI
  • decreased stereotypic behavior / MGI
  • decreased neurotransmitter release / MGI
  • abnormal striatum morphology / MGI
  • abnormal synaptic plasticity / MGI
  • abnormal spatial working memory / MGI
  • enhanced behavioral response to cocaine / MGI
  • impaired behavioral response to cocaine / MGI
  • abnormal dendritic spine morphology / MGI
  • abnormal hippocampus pyramidal cell morphology / MGI
  • increased grip strength / MGI
  • decreased grip strength / MGI
  • increased response to stress-induced hyperthermia / MGI
  • reduced AMPA-mediated synaptic currents / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • decreased vocalization / MGI

Literature references

  • Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins.;Fernández Esperanza, Collins Mark O, Uren Rachel T, Kopanitsa Maksym V, Komiyama Noboru H, Croning Mike D R, Zografos Lysimachos, Armstrong J Douglas, Choudhary Jyoti S, Grant Seth G N, ;2009;Molecular systems biology;5;269; 19455133

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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