| Status | Available to order |
| EMMA ID | EM:15869 |
| Citation information | RRID:IMSR_EM:15869 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J;129-MpdzGt(XG734)Byg/WtsiH |
| Alternative name | B6J;129-Mpdz |
| Strain type | Gene-trap |
| Allele/Transgene symbol | MpdzGt(XG734)Byg |
| Gene/Transgene symbol | Mpdz |
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information submitted by depositor: This strain displays issues with morbidity in homozygotes. The phenotype is on heterozygotes but they can generate homozygotes in timed matings. Maintain on a C57BL/6J background. Information sourced from supplementary data for https://doi.org/10.1101/500389: This strain carries the gene-trapping vector pGT1lxf was used to insert the mutation in ES cell line XG734. The insertional mutation was designed to create an in-frame fusion between the 5' exons of the trapped gene and a reporter, a fusion of beta-galactosidase and neomycin phosphotransferase. Thus, the gene-trapped locus is predicted to yield a fusion transcript containing exons 1-11 of Mpdz and beta-galactosidase. Western blots of brain extracts from knockout homozygote pups confirmed the deletion and absence of full length encoded protein |
| Phenotypic information | Homozygous:Homozygous displayed increased perinatal mortality rates from birth to 4 weeks but tissue was collected for post mortem genotypingHeterozygous:Heterozygous mice consumed significantly less of 6%, 10%, and 20% ethanol solutions per kilogram body weight each day compared to wildtype littermates. |
| Breeding history | Backcrosses onto the C57BL/6 background were used to maintain the colony and to generate heterozygous and wildtype mice to study. Genomic composition of the majority of mice used in experiments was approximately 25% 129P2/OlaHsd and 75% C57BL/6 |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | no |
| Homozygous matings required | not known |
| Immunocompromised | not known |
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
