STOCK Tg(Pou5f1-EGFP)2Mnn Tg(Tet1-mCherry)B37Koh/Orl
| Status | Available to order |
| EMMA ID | EM:15883 |
| Citation information | RRID:IMSR_EM:15883 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Tg(Pou5f1-EGFP)2Mnn Tg(Tet1-mCherry)B37Koh/Orl |
| Alternative name | B6;129S-Tg(Tet1-mCherry)B37Koh; Tg(Pou5f1-EGFP)2Mnn/J |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Tet1-mCherry)B37Koh, Tg(Pou5f1-EGFP)2Mnn |
| Gene/Transgene symbol | Tg(Tet1-mCherry)B37Koh, Tg(Pou5f1-EGFP)2Mnn |
Information from provider
| Provider | Kian Koh |
| Provider affiliation | KU Leuven |
| Genetic information | Transgene 1: the transgene contains the mCherry 2A reporter-puromycin resistance ORF cassette under the control of the distal promoter (6 kb genomic region B) of the Tet1 gene. The transgene has been introduced by stable transfection into v6.5 (C57BL/6 x 129Sv F1 hybrid) mouse embryonic stem cells. Southern blot and targeted loci amplification has verified single locus integration of the transgene at an intragenic site on chr. 10. The transgenic mouse strain was created by blastocyst injection of the clonal ES cell line number 37. Two transgenic lines (37.1 and 37.2) have been established from two founder chimeric males. Transgene 2: random transgene insertion introduced by pro-nuclei injection in (CBA/CaJ x C57BL/6J)F2 hybrid zygotes. The transgene contains an enhanced green fluorescent protein reporter gene under the control of the promoter and distal enhancer of the POU domain, class 5, transcription factor 1 (Pou5f1). |
| Phenotypic information | Homozygous:This strain harbors two randomly integrated transgenes that neither disrupt the endogenous gene nor affect embryonic development, and can be bred to homozygosity without any adverse phenotype.Heterozygous:Mice are normal. |
| Breeding history | The double transgenic strain was bred to homozygosity and sperm cryopreserved in 2018. No viable mice are kept since 2020. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
Literature references
- Regulatory Dynamics of Tet1 and Oct4 Resolve Stages of Global DNA Demethylation and Transcriptomic Changes in Reprogramming.;Bartoccetti Michela, van der Veer Bernard K, Luo Xinlong, Khoueiry Rita, She Pinyi, Bajaj Manmohan, Xu Jiayi, Janiszewski Adrian, Thienpont Bernard, Pasque Vincent, Koh Kian Peng, ;2020;Cell reports;30;2150-2169.e9; 32075734
- Allele-specific expression of imprinted genes in mouse migratory primordial germ cells.;Szabó Piroska E, Hübner Karin, Schöler Hans, Mann Jeffrey R, ;2002;Mechanisms of development;115;157-60; 12049782
- Dynamic switching of active promoter and enhancer domains regulates Tet1 and Tet2 expression during cell state transitions between pluripotency and differentiation.;Sohni Abhishek, Bartoccetti Michela, Khoueiry Rita, Spans Lien, Vande Velde Joris, De Troyer Linde, Pulakanti Kirthi, Claessens Frank, Rao Sridhar, Koh Kian Peng, ;2015;Molecular and cellular biology;35;1026-42; 25582196